Plant Physiol. Illumina
HOME HELP FEEDBACK SUBSCRIPTIONS ARCHIVE SEARCH TABLE OF CONTENTS
QUICK SEARCH:   [advanced]


     

This Article
Right arrow Full Text
Right arrow Full Text (PDF)
Right arrow Alert me when this article is cited
Right arrow Alert me if a correction is posted
Services
Right arrow Email this article to a friend
Right arrow Similar articles in this journal
Right arrow Similar articles in Web of Science
Right arrow Similar articles in PubMed
Right arrow Alert me to new issues of the journal
Right arrow Download to citation manager
Right arrow reprints & permissions
Citing Articles
Right arrow Citing Articles via HighWire
Right arrow Citing Articles via CrossRef
Right arrow Citing Articles via Web of Science (52)
Right arrow Citing Articles via Google Scholar
Google Scholar
Right arrow Articles by Sunako, T.
Right arrow Articles by Harada, T.
Right arrow Search for Related Content
PubMed
Right arrow PubMed Citation
Right arrow Articles by Sunako, T.
Right arrow Articles by Harada, T.
Agricola
Right arrow Articles by Sunako, T.
Right arrow Articles by Harada, T.

An Allele of the Ripening-Specific 1-Aminocyclopropane-1-Carboxylic Acid Synthase Gene (ACS1) in Apple Fruit with a Long Storage Life1

Tomomi Sunako, Wakako Sakuraba, Mineo Senda, Shinji Akada, Ryuji Ishikawa, Minoru Niizeki, and Takeo Harada*

Laboratory of Plant Breeding and Genetics, Faculty of Agriculture and Life Science (T.S., W.S., R.I., M.N., T.H.), and Gene Research Center (M.S., S.A.), Hirosaki University, Bunkyo-cho 3, Hirosaki 036-8561, Japan

An allele of the 1-aminocyclopropane-1-carboxylic acid (ACC) synthase gene (Md-ACS1), the transcript and translated product of which have been identified in ripening apples (Malus domestica), was isolated from a genomic library of the apple cultivar, Golden Delicious. The predicted coding region of this allele (ACS1-2) showed that seven nucleotide substitutions in the corresponding region of ACS1-1 resulted in just one amino acid transition. A 162-bp sequence characterized as a short interspersed repetitive element retrotransposon was inserted in the 5'-flanking region of ACS1-2 corresponding to position -781 in ACS1-1. The XhoI site located near the 3' end of the predicted coding region of ACS1-2 was absent from the reverse transcriptase-polymerase chain reaction product, revealing that exclusive transcription from ACS1-1 occurs during ripening of cv Golden Delicious fruit. DNA gel-blot and polymerase chain reaction analyses of genomic DNAs showed clearly that apple cultivars were either heterozygous for ACS1-1 and ACS1-2 or homozygous for each type. RNA gel-blot analysis of the ACS1-2 homozygous Fuji apple, which produces little ethylene and has a long storage life, demonstrated that the level of transcription from ACS1-2 during the ripening stage was very low.

1   This work was supported in part by a Grant-in-Aid from the Ministry of Education, Science and Culture, Japan.
*   Corresponding author; e-mail tharada @cc.hirosaki-u.ac.jp; fax 81-172-39-3750.

Plant Physiol. (1999) 119: 1297-1304
Copyright Clearance Center:   0032-0889/99/119//08
© 1999 American Society of Plant Physiologists




This article has been cited by other articles:


Home page
 Plant Physiol.Home page
A. Wang, J. Yamakake, H. Kudo, Y. Wakasa, Y. Hatsuyama, M. Igarashi, A. Kasai, T. Li, and T. Harada
Null Mutation of the MdACS3 Gene, Coding for a Ripening-Specific 1-Aminocyclopropane-1-Carboxylate Synthase, Leads to Long Shelf Life in Apple Fruit
Plant Physiology, September 1, 2009; 151(1): 391 - 399.
[Abstract] [Full Text] [PDF]

Home page
 J Exp BotHome page
A. Wang, D. Tan, A. Takahashi, T. Zhong Li, and T. Harada
MdERFs, two ethylene-response factors involved in apple fruit ripening
J. Exp. Bot., October 1, 2007; 58(13): 3743 - 3748.
[Abstract] [Full Text] [PDF]

Home page
 Plant Physiol.Home page
R. J. Schaffer, E. N. Friel, E. J.F. Souleyre, K. Bolitho, K. Thodey, S. Ledger, J. H. Bowen, J.-H. Ma, B. Nain, D. Cohen, et al.
A Genomics Approach Reveals That Aroma Production in Apple Is Controlled by Ethylene Predominantly at the Final Step in Each Biosynthetic Pathway
Plant Physiology, August 1, 2007; 144(4): 1899 - 1912.
[Abstract] [Full Text] [PDF]

Home page
 J Exp BotHome page
M. Tatsuki, T. Haji, and M. Yamaguchi
The involvement of 1-aminocyclopropane-1-carboxylic acid synthase isogene, Pp-ACS1, in peach fruit softening
J. Exp. Bot., March 1, 2006; 57(6): 1281 - 1289.
[Abstract] [Full Text] [PDF]


HOME HELP FEEDBACK SUBSCRIPTIONS ARCHIVE SEARCH TABLE OF CONTENTS
ASPB Publications PLANT PHYSIOLOGY® THE PLANT CELL
Copyright © 1999 by the American Society of Plant Biologists