|
Changes in Phosphoinositide Metabolism with Days
in Culture
Affect Signal Transduction Pathways in
Galdieria
sulphuraria1
Ingo Heilmann,
Imara Y. Perera,
Wolfgang Gross, and
Wendy F. Boss*
Department of Botany, North Carolina State University, Raleigh,
North Carolina 27695-7612 (I.H., I.Y.P., W.F.B.); and Institut
für Pflanzenphysiologie und Mikrobiologie, Freie
Universität Berlin, Königin-Luise-Strasse 12-16, 14195 Berlin, Germany (I.H., W.G.)
The metabolism of
phosphatidylinositol-4,5-bisphosphate (PIP2) changed during
the culture period of the thermoacidophilic red alga Galdieria
sulphuraria. Seven days after inoculation, the amount of
PIP2 in the cells was 910 ± 100 pmol g 1
fresh weight; by 12 d, PIP2 levels increased to
1200 ± 150 pmol g1 fresh weight. In vitro assays
indicated that phosphatidylinositol monophosphate (PIP) kinase specific
activity increased from 75 to 230 pmol min1
mg1 protein between d 7 and 12. When G. sulphuraria cells were osmostimulated, transient increases of
up to 4-fold could be observed in inositol-1,4,5-trisphosphate (IP3) levels within 90 s, regardless of the age of the
cells. In d-12 cells, the increase in IP3 was preceded by a
transient increase of up to 5-fold in specific PIP kinase activity,
whereas no such increase was detected after osmostimulation of d-7
cells. The increase in PIP kinase activity before IP3
signaling in d-12 cells indicates that there is an additional pathway
for regulation of phosphoinositide metabolism after stimulation other
than an initial activation of phospholipase C. Also, the rapid
activation of PIP2 biosynthesis in cells with already-high
PIP2 levels suggests that the PIP2 present was
not available for signal transduction. By comparing the response of the
cells at d 7 and 12, we have identified two potentially distinct pools
of PIP2.
1
This research was supported by the National
Aeronautics and Space Administration (grant no. NAGW-4984 to W.F.B.)
and a Deutscher Akademischer Austauschdienst fellowship (HSP III to
I.H.) financed by the German Federal Ministry of Education, Science,
Research, and Technology.
*
Corresponding author; e-mail wendy_boss{at}ncsu.edu; fax
1-919-515-3436.
Plant Physiol. (1999) 119: 1331-1340
Copyright Clearance Center: 0032-0889/99/119//10
© 1999 American Society of Plant Physiologists
This article has been cited by other articles:
|
|
|
|
 
A. Mosblech, S. Konig, I. Stenzel, P. Grzeganek, I. Feussner, and I. Heilmann
Phosphoinositide and Inositolpolyphosphate Signalling in Defense Responses of Arabidopsis thaliana Challenged by Mechanical Wounding
Mol Plant,
March 1, 2008;
1(2):
249 - 261.
[Abstract]
[Full Text]
[PDF]
|
|
|
|
|
|
|
I. Stenzel, T. Ischebeck, S. Konig, A. Holubowska, M. Sporysz, B. Hause, and I. Heilmann
The Type B Phosphatidylinositol-4-Phosphate 5-Kinase 3 Is Essential for Root Hair Formation in Arabidopsis thaliana
PLANT CELL,
January 1, 2008;
20(1):
124 - 141.
[Abstract]
[Full Text]
[PDF]
|
|
|
|
|
|
|
S. Konig, A. Mosblech, and I. Heilmann
Stress-inducible and constitutive phosphoinositide pools have distinctive fatty acid patterns in Arabidopsis thaliana
FASEB J,
July 1, 2007;
21(9):
1958 - 1967.
[Abstract]
[Full Text]
[PDF]
|
|
|
|
|
|
|
L. Zonia and T. Munnik
Osmotically Induced Cell Swelling versus Cell Shrinking Elicits Specific Changes in Phospholipid Signals in Tobacco Pollen Tubes
Plant Physiology,
February 1, 2004;
134(2):
813 - 823.
[Abstract]
[Full Text]
[PDF]
|
|
|
|
|
|
|
I. Y. Perera, J. Love, I. Heilmann, W. F. Thompson, and W. F. Boss
Up-Regulation of Phosphoinositide Metabolism in Tobacco Cells Constitutively Expressing the Human Type I Inositol Polyphosphate 5-Phosphatase
Plant Physiology,
August 1, 2002;
129(4):
1795 - 1806.
[Abstract]
[Full Text]
[PDF]
|
|
|
|
|
|
|
L. Xiong, B.-h. Lee, M. Ishitani, H. Lee, C. Zhang, and J.-K. Zhu
FIERY1 encoding an inositol polyphosphate 1-phosphatase is a negative regulator of abscisic acid and stress signaling in Arabidopsis
Genes & Dev.,
August 1, 2001;
15(15):
1971 - 1984.
[Abstract]
[Full Text]
[PDF]
|
|
|
|
|
|
|
I. Heilmann, I. Y. Perera, W. Gross, and W. F. Boss
Plasma Membrane Phosphatidylinositol 4,5-Bisphosphate Levels Decrease with Time in Culture
Plant Physiology,
August 1, 2001;
126(4):
1507 - 1518.
[Abstract]
[Full Text]
[PDF]
|
|
|
|
|
|
|
I. Y. Perera, I. Heilmann, S. C. Chang, W. F. Boss, and P. B. Kaufman
A Role for Inositol 1,4,5-Trisphosphate in Gravitropic Signaling and the Retention of Cold-Perceived Gravistimulation of Oat Shoot Pulvini
Plant Physiology,
March 1, 2001;
125(3):
1499 - 1507.
[Abstract]
[Full Text]
|
|
|
|
|
