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Proline Accumulation in Maize (Zea mays L.)
Primary Roots at Low Water Potentials. II. Metabolic Source of
Increased Proline Deposition in the Elongation Zone1
Paul E. Verslues2 and
Robert E. Sharp*
Department of Agronomy, Plant Science Unit, 1-87 Agriculture
Building, University of Missouri, Columbia, Missouri 65211
The
proline (Pro) concentration increases greatly in the growing region of
maize (Zea mays L.) primary roots at low water potentials ( w), largely as a result of an increased net
rate of Pro deposition. Labeled glutamate (Glu), ornithine
(Orn), or Pro was supplied specifically to the root tip of intact
seedlings in solution culture at high and low w to
assess the relative importance of Pro synthesis, catabolism,
utilization, and transport in root-tip Pro deposition. Labeling with
[3H]Glu indicated that Pro synthesis from Glu did not
increase substantially at low w and accounted for only a
small fraction of the Pro deposition. Labeling with
[14C]Orn showed that Pro synthesis from Orn also could
not be a substantial contributor to Pro deposition. Labeling with
[3H]Pro indicated that neither Pro catabolism nor
utilization in the root tip was decreased at low w. Pro
catabolism occurred at least as rapidly as Pro synthesis from Glu.
There was, however, an increase in Pro uptake at low w,
which suggests increased Pro transport. Taken together, the data
indicate that increased transport of Pro to the root tip serves as the
source of low-w-induced Pro accumulation. The possible
significance of Pro catabolism in sustaining root growth at low
w is also discussed.
1
This work was supported by National Science
Foundation grant no. IBN-9306935 to R.E.S. and Eric S. Ober. P.E.V. was
supported by a fellowship from the University of Missouri Maize Biology Training Program, a unit of the Department of Energy/National Science
Foundation/U.S. Department of Agriculture Collaborative Research in
Plant Biology Program (grant no. BIR-9420688). This is contribution no.
12,859 from the Missouri Agricultural Experiment Station journal
series.
2
Present address: Department of Botany and Plant
Sciences, University of California, Riverside, CA 92521.
*
Corresponding author; e-mail sharpr{at}missouri.edu; fax
1-573-882-1469.
Plant Physiol. (1999) 119: 1349-1360
Copyright Clearance Center: 0032-0889/99/119//12
© 1999 American Society of Plant Physiologists
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