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Cloning and Characterization of a Gibberellin-Induced RNase Expressed in Barley Aleurone Cells1

Sally W. Rogers* and John C. Rogers

Institute of Biological Chemistry, Washington State University, Pullman, Washington 99164-6340

We cloned a cDNA for a gibberellin-induced ribonuclease (RNase) expressed in barley (Hordeum vulgare) aleurone and the gene for a second barley RNase expressed in leaf tissue. The protein encoded by the cDNA is unique among RNases described to date in that it contains a novel 23-amino acid insert between the C2 and C3 conserved sequences. Expression of the recombinant protein in tobacco (Nicotiana tabacum) suspension-cultured protoplasts gave an active RNase of the expected size, confirming the enzymatic activity of the protein. Analyses of hormone regulation of expression of mRNA for the aleurone RNase revealed that, like the pattern for alpha -amylase, mRNA levels increased in the presence of gibberellic acid, and its antagonist abscisic acid prevented this effect. Quantitative studies at early times demonstrated that cycloheximide treatment of aleurone layers increased mRNA levels 4-fold, whereas a combination of gibberellin plus cycloheximide treatment was required to increase alpha -amylase mRNA levels to the same extent. These results are consistent with loss of repression as an initial effect of gibberellic acid on transcription of those genes, although the regulatory pathways for the two genes may differ.

1   This research was supported by the Department of Energy (grant no. DE-FG 95ER 20165).
*   Corresponding author; e-mail bcsroger{at}wsu.edu; fax 1-509-335-7643.

Plant Physiol. (1999) 119: 1457-1464
Copyright Clearance Center:   0032-0889/99/119//08
© 1999 American Society of Plant Physiologists




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A. Lers, L. Sonego, P. J. Green, and S. Burd
Suppression of LX Ribonuclease in Tomato Results in a Delay of Leaf Senescence and Abscission
Plant Physiology, October 1, 2006; 142(2): 710 - 721.
[Abstract] [Full Text] [PDF]


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