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A Gene Encoding a Hevein-Like Protein from Elderberry Fruits Is Homologous to PR-4 and Class V Chitinase Genes1

Els J.M. Van Damme*, Diana Charels, Soma Roy, Koenraad Tierens, Annick Barre, José C. Martins, Pierre Rougé, Fred Van Leuven, Mirjam Does, and Willy J. Peumans

Laboratory for Phytopathology and Plant Protection, Willem de Croylaan 42 (E.J.M.V.D., D.C., S.R., W.J.P.), F.A. Janssens Laboratory of Genetics, Kardinaal Mercierlaan 92 (K.T.), and Center for Human Genetics, Herestraat 49 (F.V.L.), Katholieke Universiteit Leuven, 3001 Leuven, Belgium; Katholieke Universiteit Leuven, 3001 Leuven, BelgiumInstitut de Pharmacologie et Biologie Structurale, Unité Propre de Recherche Centre National de la Recherche Scientifique 9062, 205 Route de Narbonne, 31077 Toulouse cedex, France (A.B., P.R.); High Resolution NMR Centre, Vrije Universiteit Brussel, Pleinlaan 2, 1050 Brussels, Belgium (J.C.M.); and Section of Plant Pathology, Institute of Molecular Cell Biology, BioCentrum Amsterdam, Kruislaan 318, 1098 SM Amsterdam, The Netherlands (M.D.)

We isolated SN-HLPf (Sambucus nigra hevein-like fruit protein), a hevein-like chitin-binding protein, from mature elderberry fruits. Cloning of the corresponding gene demonstrated that SN-HLPf is synthesized as a chimeric precursor consisting of an N-terminal chitin-binding domain corresponding to the mature elderberry protein and an unrelated C-terminal domain. Sequence comparisons indicated that the N-terminal domain of this precursor has high sequence similarity with the N-terminal domain of class I PR-4 (pathogenesis-related) proteins, whereas the C terminus is most closely related to that of class V chitinases. On the basis of these sequence homologies the gene encoding SN-HLPf can be considered a hybrid between a PR-4 and a class V chitinase gene.

1   This work was supported in part by the Katholieke Universiteit Leuven (grant no. OT/94/17), by the Centre National de la Recherche Scientifique, by the Conseil Régional de Midi-Pyrénées, by the Fonds voor Wetenschappelijk Onderzoek-Levenslijn fund (grant no. 7-0047-90), and by the Fund for Scientific Research-Flanders (grant no. G-0223-97). W.P. is the research director and E.V.D. and J.C.M. are postdoctoral fellows of the Fund for Scientific Research-Flanders. K.T. acknowledges the receipt of a fellowship from the Vlaams Instituut voor de bevordering van het Wetenschappelijk-Technologisch Onderzoek in de Industrie.
*   Corresponding author; e-mail els.vandamme{at}agr.kuleuven.ac.be; fax 32-16-322976.

Plant Physiol. (1999) 119: 1547-1556
Copyright Clearance Center:   0032-0889/99/119//10
© 1999 American Society of Plant Physiologists




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