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The MADS-Domain Protein AGAMOUS-Like 15 Accumulates in Embryonic Tissues with Diverse Origins1
Sharyn E. Perry2,
Melissa D. Lehti, and
Donna E. Fernandez*
Department of Botany, University of Wisconsin, 430 Lincoln Drive,
Madison, Wisconsin 53706-1381
AGL15
(AGAMOUS-like 15), a member of the
MADS-domain family of regulatory factors, accumulates preferentially in
the organs and tissues derived from double fertilization in flowering
plants (i.e. the embryo, suspensor, and endosperm). The developmental role of AGL15 is still undefined. If it is involved in embryogenesis rather than some other aspect of seed biology, then AGL15 protein should accumulate whenever development proceeds in the embryonic mode,
regardless of the origin of those embryos or their developmental context. To test this, we used AGL15-specific antibodies to analyze apomictic embryogenesis in dandelion (Taraxacum
officinale), microspore embryogenesis in oilseed rape
(Brassica napus), and somatic embryogenesis in alfalfa
(Medicago sativa). In every case, AGL15 accumulated to
relatively high levels in the nuclei of the embryos. AGL15 also
accumulated in cotyledon-like organs produced by the
xtc2 (extra
cotyledon2)
mutant of Arabidopsis and during precocious germination in oilseed
rape. Furthermore, the subcellular localization of AGL15 appeared to be
developmentally regulated in all embryogenic situations. AGL15 was
initially present in the cytoplasm of cells and became nuclear
localized before or soon after embryogenic cell divisions began. These
results support the hypothesis that AGL15 participates in the
regulation of programs active during the early stages of embryo
development.
1
This research was supported by grants to the
University of Wisconsin from the U.S. Department of Energy/National
Science Foundation/U.S. Department of Agriculture Collaborative Program
on Research in Plant Biology (no. BIR 92-20331), by the National
Science Foundation (grant no. BIR-9403929 to S.E.P.), and by the U.S.
Department of Agriculture (grant no. 9602636 to D.E.F.). M.D.L. was
supported in part by a National Institutes of Health training
fellowship (no. NIH-5-T32-GM08349).
2
Present address: Department of Agronomy,
University of Kentucky, Lexington, KY 40546-0091.
*
Corresponding author; e-mail dfernand{at}facstaff.wisc.edu; fax
1-608-262-7509.
Plant Physiol. (1999) 120: 121-130
Copyright Clearance Center: 0032-0889/99/120//10
© 1999 American Society of Plant Physiologists
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