Plant Physiol. Illumina
HOME HELP FEEDBACK SUBSCRIPTIONS ARCHIVE SEARCH TABLE OF CONTENTS
QUICK SEARCH:   [advanced]


     

This Article
Right arrow Full Text
Right arrow Full Text (PDF)
Right arrow Alert me when this article is cited
Right arrow Alert me if a correction is posted
Services
Right arrow Email this article to a friend
Right arrow Similar articles in this journal
Right arrow Similar articles in Web of Science
Right arrow Similar articles in PubMed
Right arrow Alert me to new issues of the journal
Right arrow Download to citation manager
Right arrow reprints & permissions
Citing Articles
Right arrow Citing Articles via HighWire
Right arrow Citing Articles via CrossRef
Right arrow Citing Articles via Web of Science (26)
Right arrow Citing Articles via Google Scholar
Google Scholar
Right arrow Articles by Wolkers, W. F.
Right arrow Articles by Hoekstra, F. A.
Right arrow Search for Related Content
PubMed
Right arrow PubMed Citation
Right arrow Articles by Wolkers, W. F.
Right arrow Articles by Hoekstra, F. A.
Agricola
Right arrow Articles by Wolkers, W. F.
Right arrow Articles by Hoekstra, F. A.

Changed Properties of the Cytoplasmic Matrix Associated with Desiccation Tolerance of Dried Carrot Somatic Embryos. An in Situ Fourier Transform Infrared Spectroscopic Study1

Willem F. Wolkers2, Frans A.A. Tetteroo, Mark Alberda, and Folkert A. Hoekstra

Laboratory of Plant Physiology, Wageningen Agricultural University, Arboretumlaan 4, NL-6703 BD Wageningen, The Netherlands (W.F.W., M.A., F.A.H.); and Incotec, Westeinde 107, NL-1601 BL Enkhuizen, The Netherlands (F.A.A.T.)

Abscisic acid-pretreated carrot (Daucus carota) somatic embryos survive dehydration upon slow drying, but fast drying leads to poor survival of the embryos. To determine whether the acquisition of desiccation tolerance is associated with changes in the physical stability of the cytoplasm, in situ Fourier transform infrared microspectroscopy was used. Although protein denaturation temperatures were similar in the embryos after slow or fast drying, the extent of the denaturation was greater after fast drying. Slowly dried embryos are in a glassy state at room temperature, and no clearly defined glassy matrix was observed in the rapidly dried embryos. At room temperature the average strength of hydrogen bonding was much weaker in the rapidly dried than in the slowly dried embryos. We interpreted the molecular packing to be "less tight" in the rapidly dried embryos. Whereas sucrose (Suc) is the major soluble carbohydrate after fast drying, upon slow drying the trisaccharide umbelliferose accumulates at the expense of Suc. The possibly protective role of umbelliferose was tested on protein and phospholipid model systems, using Suc as a reference. Both umbelliferose and Suc form a stable glass with drying: They depress the transition temperature of dry liposomal membranes equally well, they both prevent leakage from dry liposomes after rehydration, and they protect a polypeptide that is desiccation sensitive. The similar protection properties in model systems and the apparent interchangeability of both sugars in viable, dry somatic embryos suggest no special role of umbelliferose in the improved physical stability of the slowly dried embryos. Also, during slow drying LEA (late-embryogenesis abundant) transcripts are expressed. We suggest that LEA proteins embedded in the glassy matrix confer stability to these slowly dried embryos.

1   This project was financially supported by the Life Sciences Foundation, which is subsidized by the Netherlands Organization for Scientific Research.
2   Present address: Section of Molecular and Cellular Biology, University of California, Davis, CA 95616.

Plant Physiol. (1999) 120: 153-164
Copyright Clearance Center:   0032-0889/99/120//12
© 1999 American Society of Plant Physiologists




This article has been cited by other articles:


Home page
 Proc. Natl. Acad. Sci. USAHome page
M. Sakurai, T. Furuki, K.-i. Akao, D. Tanaka, Y. Nakahara, T. Kikawada, M. Watanabe, and T. Okuda
Vitrification is essential for anhydrobiosis in an African chironomid, Polypedilum vanderplanki
PNAS, April 1, 2008; 105(13): 5093 - 5098.
[Abstract] [Full Text] [PDF]

Home page
 Integr. Comp. Biol.Home page
J. H. Crowe, L. M. Crowe, W. F. Wolkers, A. E. Oliver, X. Ma, J.-H. Auh, M. Tang, S. Zhu, J. Norris, and F. Tablin
Stabilization of Dry Mammalian Cells: Lessons from Nature
Integr. Comp. Biol., November 1, 2005; 45(5): 810 - 820.
[Abstract] [Full Text] [PDF]

Home page
 J Exp BotHome page
V. V. Terskikh, J. A. Feurtado, S. Borchardt, M. Giblin, S. R. Abrams, and A. R. Kermode
In vivo 13C NMR metabolite profiling: potential for understanding and assessing conifer seed quality
J. Exp. Bot., August 1, 2005; 56(418): 2253 - 2265.
[Abstract] [Full Text] [PDF]

Home page
 Plant Physiol.Home page
J. Yang and H. E. Yen
Early Salt Stress Effects on the Changes in Chemical Composition in Leaves of Ice Plant and Arabidopsis. A Fourier Transform Infrared Spectroscopy Study
Plant Physiology, October 1, 2002; 130(2): 1032 - 1042.
[Abstract] [Full Text] [PDF]

Home page
 ANN BOT (LOND)Home page
L. SREEDHAR, W. F. WOLKERS, F. A. HOEKSTRA, and J. D. BEWLEY
In vivo Characterization of the Effects of Abscisic Acid and Drying Protocols Associated with the Acquisition of Desiccation Tolerance in Alfalfa (Medicago sativa L.) Somatic Embryos
Ann. Bot., April 1, 2002; 89(4): 391 - 400.
[Abstract] [Full Text] [PDF]

Home page
 Plant Physiol.Home page
O. Leprince, F. J.M. Harren, J. Buitink, M. Alberda, and F. A. Hoekstra
Metabolic Dysfunction and Unabated Respiration Precede the Loss of Membrane Integrity during Dehydration of Germinating Radicles
Plant Physiology, February 1, 2000; 122(2): 597 - 608.
[Abstract] [Full Text]


HOME HELP FEEDBACK SUBSCRIPTIONS ARCHIVE SEARCH TABLE OF CONTENTS
ASPB Publications PLANT PHYSIOLOGY® THE PLANT CELL
Copyright © 1999 by the American Society of Plant Biologists