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Plastid Sedimentation Kinetics in Roots of Wild-Type and
Starch-Deficient Mutants of Arabidopsis1
Scott A. MacCleery and
John Z. Kiss*
Department of Botany, Miami University, Oxford, Ohio 45056
Sedimentation and movement of
plastids in columella cells of the root cap were measured in seedlings
of wild-type, a reduced starch mutant, and a starchless mutant of
Arabidopsis. To assay for sedimentation, we used both linear
measurements and the change of angle from the cell center as indices in
vertical and reoriented plants with the aid of computer-assisted image
analysis. Seedlings were fixed at short periods after reorientation,
and plastid sedimentation correlated with starch content in the three
strains of Arabidopsis. Amyloplasts of wild-type seedlings showed the
greatest sedimentation, whereas plastids of the starchless mutant
showed no significant sedimentation in the vertically grown and
reoriented seedlings. Because previous research has shown that a full
complement of starch is needed for full gravitropic sensitivity, this
study correlates increased sensitivity with plastid sedimentation.
However, although plastid sedimentation contributed to
gravisensitivity, it was not required, because the gravitropic
starchless mutant had plastids that did not sediment. This is the first
study, to our knowledge, to measure plastid sedimentation in
Arabidopsis roots after reorientation of seedlings. Taken together, the
results of this study are consistent with the classic plastid-based and protoplast-based models of graviperception and suggest that multiple systems of perception exist in plant cells.
1
This research was supported by the National
Aeronautics and Space Administration (grant no. NAG 2-1017).
*
Corresponding author; e-mail kissjz{at}muohio.edu; fax
1-513-529-4243.
Plant Physiol. (1999) 120: 183-192
Copyright Clearance Center: 0032-0889/99/120//10
© 1999 American Society of Plant Physiologists
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