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Greening under High Light or Cold Temperature Affects the Level
of Xanthophyll-Cycle Pigments, Early Light-Inducible Proteins, and
Light-Harvesting Polypeptides in Wild-Type Barley and the
Chlorina f2 Mutant1
Marianna Król,
Alexander G. Ivanov,
Stefan Jansson,
Klaus Kloppstech, and
Norman P.A. Huner*
Department of Plant Sciences, The University of Western Ontario,
London, Ontario, Canada N6A 5B7 (M.K., A.G.I., N.P.A.H.); Department of Plant Physiology, University of Umeå, S 901 87 Umeå,
Sweden (S.J.); and Institut für Botanik, Universität
Hannover, Herrnhäuser Strasse 2, Hannover 21 3000, Germany (K.K.)
Etiolated seedlings of wild type and
the chlorina f2 mutant of barley (Hordeum
vulgare) were exposed to greening at either 5°C or 20°C and
continuous illumination varying from 50 to 800 µmol m 2
s1. Exposure to either moderate temperature and high
light or low temperature and moderate light inhibited chlorophyll
a and b accumulation in the wild type and
in the f2 mutant. Continuous illumination under these
greening conditions resulted in transient accumulations of zeaxanthin,
concomitant transient decreases in violaxanthin, and fluctuations in
the epoxidation state of the xanthophyll pool. Photoinhibition-induced
xanthophyll-cycle activity was detectable after only 3 h of
greening at 20°C and 250 µmol m2 s1.
Immunoblot analyses of the accumulation of the 14-kD early
light-inducible protein but not the major (Lhcb2) or minor (Lhcb5)
light-harvesting polypeptides demonstrated transient kinetics similar
to those observed for zeaxanthin accumulation during greening at either 5°C or 20°C for both the wild type and the f2
mutant. Furthermore, greening of the f2 mutant at either
5°C or 20°C indicated that Lhcb2 is not essential for the
regulation of the xanthophyll cycle in barley. These results are
consistent with the thesis that early light-inducible proteins may bind
zeaxanthin as well as other xanthophylls and dissipate excess light
energy to protect the developing photosynthetic apparatus from excess
excitation. We discuss the role of energy balance and photosystem II
excitation pressure in the regulation of the xanthophyll cycle during
chloroplast biogenesis in wild-type barley and the f2
mutant.
1
This research was supported by the Natural
Sciences and Engineering Research Council of Canada (grant to
N.P.A.H.), by the Swedish Forestry and Agriculture Research Council
(grant to S.J.), and by the Deutsche Forschungsgemeinschaft (grant to
K.K.).
*
Corresponding author; e-mail nhuner{at}julian.uwo.ca; fax
1-519-661-3935.
Plant Physiol. (1999) 120: 193-204
Copyright Clearance Center: 0032-0889/99/120//12
© 1999 American Society of Plant Physiologists
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