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Messenger RNA-Binding Properties of Nonpolysomal Ribonucleoproteins from Heat-Stressed Tomato Cells1

Rogier Stuger2, Sigrid Ranostaj, Tilo Materna3, and Christoph Forreiter*

Department of Molecular Cell Biology, Goethe University, Marie Curie Strasse 9, 60439 Frankfurt am Main, Germany

Most cells experiencing heat stress reprogram their translational machinery to favor the synthesis of heat-stress proteins. Translation of other transcripts is almost completely repressed, but most untranslated messengers are not degraded. In contrast to yeast, Drosophila melanogaster, and HeLa cells, plant cells store repressed messengers in cytoplasmic nonpolysomal ribonucleoproteins (RNPs). To follow the fate of untranslated transcripts, we studied protein composition, mRNA content, and RNA-binding properties of nonpolysomal RNPs from heat-stressed tomato (Lycopersicon peruvianum) cells. Contrary to the selective interaction in vivo, RNPs isolated from tomato cells bound both stress-induced and repressed messengers, suggesting that the selection mechanism resides elsewhere. This binding was independent of a cap or a poly(A) tail. The possible role of proteasomes and heat-stress granules (HSGs) in mRNA storage is a topic of debate. We found in vitro messenger-RNA-binding activity in messenger RNP fractions free of C2-subunit-containing proteasomes and HSGs. In addition, mRNAs introduced into tobacco (Nicotiana plumbaginifolia) protoplasts were found in the cytoplasm but were not associated with HSGs.

1   This work was funded by the Deutsche Forschungsgemeinschaft (grant no. No 249/1).
2   Present address: Department of Molecular Cell Physiology, Free University, de Boelelaan 1087, 1081 HV Amsterdam, The Netherlands.
3   Present address: Genetics Unit, Novartis Forschungsinstitut, Brunnerstrasse 59, 1235 Wien, Austria.
*   Corresponding author; e-mail forreiter{at}cellbiology.unifrankfurt.de; fax 49-69-798-29286.

Plant Physiol. (1999) 120: 23-32
Copyright Clearance Center:   0032-0889/99/120//10
© 1999 American Society of Plant Physiologists




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J. Ludwig-Müller, P. Krishna, and C. Forreiter
A Glucosinolate Mutant of Arabidopsis Is Thermosensitive and Defective in Cytosolic Hsp90 Expression after Heat Stress
Plant Physiology, July 1, 2000; 123(3): 949 - 958.
[Abstract] [Full Text]


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