Plant Physiol. EPICENTRE Biotechnologies
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ATP-Dependent Formation of Phosphatidylserine-Rich Vesicles from the Endoplasmic Reticulum of Leek Cells

Bénédicte Sturbois-Balcerzak, Patrick Vincent, Lilly Maneta-Peyret, Michel Duvert, Béatrice Satiat-Jeunemaitre, Claude Cassagne, and Patrick Moreau*

Laboratoire de Biogenèse Membranaire, Unite Mixte de Recherche-5544---Centre National de la Recherche Scientifique (CNRS) (B.S.-B., P.V., L.M.-P., C.C., P.M.), Centre de Microscopie Électronique (M.D.), and Ecole Supérieure de Technologie des Biomolécules (C.C.), Université Victor Segalen Bordeaux 2, 146 rue Léo Saignat, 33076 Bordeaux cédex, France; and Université Victor Segalen Bordeaux 2, 146 rue Léo Saignat, 33076 Bordeaux cédex, FranceInstitut des Sciences Végétales, Unité Propre de Recherche 40-CNRS, 91198 Gif-sur-Yvette, France (B.S.-J.)

Leek (Allium porrum) plasma membrane is enriched in phosphatidylserine (PS) by the vesicular pathway, in a way similar to that already observed in animal cells (B. Sturbois-Balcerzak, D.J. Morré, O. Loreau, J.P. Noel, P. Moreau, C. Cassagne [1995] Plant Physiol Biochem 33: 625-637). In this paper we document the formation of PS-rich small vesicles from leek endoplasmic reticulum (ER) membranes upon addition of ATP and other factors. The omission of ATP or its replacement by ATPgamma -S prevents vesicle formation. These vesicles correspond to small structures (70-80 nm) and their phospholipid composition, characterized by a PS enrichment, is compatible with a role in PS transport. Moreover, the PS enrichment over phosphatidylinositol in the ER-derived vesicles is the first example, to our knowledge, of phospholipid sorting from the ER to ER-derived vesicles in plant cells.

*   Corresponding author; e-mail pmoreau{at}biomemb.u-bordeaux2.fr; fax 33-5-56-51-83-61.

Plant Physiol. (1999) 120: 245-256
Copyright Clearance Center:   0032-0889/99/120//12
© 1999 American Society of Plant Physiologists




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