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Membrane Lipid Integrity Relies on a Threshold of ATP Production
Rate in Potato Cell Cultures Submitted to Anoxia1
André Rawyler*,
Danijela Pavelic,
Christian Gianinazzi,
Jacques Oberson, and
Roland Braendle
Pflanzenphysiologisches Institut, Universität Bern,
Altenbergrain 21, CH-3013 Bern, Switzerland
In this paper we report on our study
of the changes in biomass, lipid composition, and fermentation end
products, as well as in the ATP level and synthesis rate in cultivated
potato (Solanum tuberosum) cells submitted to anoxia
stress. During the first phase of about 12 h, cells coped with the
reduced energy supply brought about by fermentation and their membrane
lipids remained intact. The second phase (12-24 h), during which the
energy supply dropped down to 1% to 2% of its maximal theoretical
normoxic value, was characterized by an extensive hydrolysis of
membrane lipids to free fatty acids. This autolytic process was
ascribed to the activation of a lipolytic acyl hydrolase. Cells were
also treated under normoxia with inhibitors known to interfere with
energy metabolism. Carbonyl-cyanide-4-trifluoromethoxyphenylhydrazone did not induce lipid hydrolysis, which was also the case when sodium
azide or salicylhydroxamic acid were fed separately. However, the
simultaneous use of sodium azide plus salicylhydroxamic acid or
2-deoxy-D-glucose plus iodoacetate with normoxic cells
promoted a lipid hydrolysis pattern similar to that seen in anoxic
cells. Therefore, a threshold exists in the rate of ATP
synthesis (approximately 10 µmol g 1 fresh weight
h1), below which the integrity of the membranes in anoxic
potato cells cannot be preserved.
1
This work was supported by the Swiss National
Science Foundation.
*
Corresponding author; e-mail andre.rawyler{at}pfp.unibe.ch; fax
41-31-332-2059.
Plant Physiol. (1999) 120: 293-300
Copyright Clearance Center: 0032-0889/99/120//08
© 1999 American Society of Plant Physiologists
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