|
Arabidopsis 22-Kilodalton Peroxisomal Membrane
Protein.
Nucleotide Sequence Analysis and
Biochemical
Characterization1
H. Bülent Tugal,
Martin Pool2, and
Alison Baker*
Centre for Plant Sciences, Leeds Institute for Plant Biotechnology
and Agriculture, University of Leeds, Leeds LS2 9JT, United Kingdom
We
sequenced and characterized PMP22 (22-kD
peroxisomal membrane protein) from
Arabidopsis, which shares 28% to 30% amino acid identity and 55% to
57% similarity to two related mammalian peroxisomal membrane proteins,
PMP22 and Mpv17. Subcellular fractionation studies confirmed that the
Arabidopsis PMP22 is a genuine peroxisomal membrane protein.
Biochemical analyses established that the Arabidopsis PMP22 is an
integral membrane protein that is completely embedded in the lipid
bilayer. In vitro import assays demonstrated that the protein is
inserted into the membrane posttranslationally in the absence of ATP,
but that ATP stimulates the assembly into the native state. Arabidopsis
PMP22 is expressed in all organs of the mature plant and in
tissue-cultured cells. Expression of PMP22 is not associated with a
specific peroxisome type, as it is detected in seeds and throughout
postgerminative growth as cotyledon peroxisomes undergo conversion from
glyoxysomes to leaf-type peroxisomes. Although PMP22 shows increased
accumulation during the growth of young seedlings, its expression is
not stimulated by light.
1
This work was supported by the Biotechnology and
Biology Research Council, UK (grant no. 24/C06847), and by the
Leverhulme Trust, UK (grant no. F/122/AW to A.B.).
2
Present address: Zentrum Molecular Biology
Heidelberg, Im Neuenheimerfeld 282, D69120 Heidelberg, Germany.
*
Corresponding author; e-mail a.baker{at}leeds.ac.uk; fax
44-113-233-3144.
Plant Physiol. (1999) 120: 309-320
Copyright Clearance Center: 0032-0889/99/120//12
© 1999 American Society of Plant Physiologists
This article has been cited by other articles:
|
|
|
|
 
H. Eubel, E. H. Meyer, N. L. Taylor, J. D. Bussell, N. O'Toole, J. L. Heazlewood, I. Castleden, I. D. Small, S. M. Smith, and A. H. Millar
Novel Proteins, Putative Membrane Transporters, and an Integrated Metabolic Network Are Revealed by Quantitative Proteomic Analysis of Arabidopsis Cell Culture Peroxisomes
Plant Physiology,
December 1, 2008;
148(4):
1809 - 1829.
[Abstract]
[Full Text]
[PDF]
|
|
|
|
|
|
|
F. Liu, S. K. Ng, Y. Lu, W. Low, J. Lai, and G. Jedd
Making two organelles from one: Woronin body biogenesis by peroxisomal protein sorting
J. Cell Biol.,
January 28, 2008;
180(2):
325 - 339.
[Abstract]
[Full Text]
[PDF]
|
|
|
|
|
|
|
A. Trott and K. A. Morano
SYM1 Is the Stress-Induced Saccharomyces cerevisiae Ortholog of the Mammalian Kidney Disease Gene Mpv17 and Is Required for Ethanol Metabolism and Tolerance during Heat Shock
Eukaryot. Cell,
June 1, 2004;
3(3):
620 - 631.
[Abstract]
[Full Text]
[PDF]
|
|
|
|
|
|
|
M. A. Murphy, B. A. Phillipson, A. Baker, and R. T. Mullen
Characterization of the Targeting Signal of the Arabidopsis 22-kD Integral Peroxisomal Membrane Protein
Plant Physiology,
October 1, 2003;
133(2):
813 - 828.
[Abstract]
[Full Text]
[PDF]
|
|
|
|
|
|
|
S. Lawand, A.-J. Dorne, D. Long, G. Coupland, R. Mache, and P. Carol
Arabidopsis A BOUT DE SOUFFLE, Which Is Homologous with Mammalian Carnitine Acyl Carrier, Is Required for Postembryonic Growth in the Light
PLANT CELL,
September 1, 2002;
14(9):
2161 - 2173.
[Abstract]
[Full Text]
[PDF]
|
|
|
|
|
|
|
L. A. del Rio, F. J. Corpas, L. M. Sandalio, J. M. Palma, M. Gomez, and J. B. Barroso
Reactive oxygen species, antioxidant systems and nitric oxide in peroxisomes
J. Exp. Bot.,
May 15, 2002;
53(372):
1255 - 1272.
[Abstract]
[Full Text]
[PDF]
|
|
|
|
|
|
|
U. Brosius, T. Dehmel, and J. Gartner
Two Different Targeting Signals Direct Human Peroxisomal Membrane Protein 22 to Peroxisomes
J. Biol. Chem.,
January 4, 2002;
277(1):
774 - 784.
[Abstract]
[Full Text]
|
|
|
|
|
|
|
T. L. Johnson and L. J. Olsen
Building New Models for Peroxisome Biogenesis
Plant Physiology,
November 1, 2001;
127(3):
731 - 739.
[Full Text]
[PDF]
|
|
|
|
|
|
|
Y. Fukao, Y. Hayashi, S. Mano, M. Hayashi, and M. Nishimura
Developmental Analysis of a Putative ATP/ADP Carrier Protein Localized on Glyoxysomal Membranes During the Peroxisome Transition in Pumpkin Cotyledons
Plant Cell Physiol.,
August 1, 2001;
42(8):
835 - 841.
[Abstract]
[Full Text]
[PDF]
|
|
|
|
|
|
|
F. J. Corpas, L. M. Sandalio, M. J. Brown, L. A. d. Rio, and R. N. Trelease
Identification of Porin-Like Polypeptide(s) in the Boundary Membrane of Oilseed Glyoxysomes
Plant Cell Physiol.,
November 1, 2000;
41(11):
1218 - 1228.
[Abstract]
[Full Text]
[PDF]
|
|
|
|
|
|
|
G. P. Copenhaver, K. Nickel, T. Kuromori, M. Benito, S. Kaul, X. Lin, M. Bevan, G. Murphy, B. Harris, L. D. Parnell, et al.
Genetic Definition and Sequence Analysis of Arabidopsis Centromeres
Science,
December 24, 1999;
286(5449):
2468 - 2474.
[Abstract]
[Full Text]
|
|
|
|
|
|
|
R. T. Mullen and R. N. Trelease
The Sorting Signals for Peroxisomal Membrane-bound Ascorbate Peroxidase Are within Its C-terminal Tail
J. Biol. Chem.,
May 19, 2000;
275(21):
16337 - 16344.
[Abstract]
[Full Text]
[PDF]
|
|
|
|
|
