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Heterologous Expression of Arabidopsis Phytochrome B in
Transgenic Potato Influences Photosynthetic Performance and Tuber
Development1
Alexandra Thiele,
Michael Herold,
Ingo Lenk,
Peter H. Quail, and
Christiane Gatz*
Albrecht von Haller Institut für Pflanzenwissenschaften,
Untere Karspüle 2, Georg August Universität
Göttingen, 37073 Göttingen, Germany (A.T., M.H., I.L.,
C.G.); and and University of California (Berkeley)/United
States Department of Agriculture Plant Gene Expression Center, 800 Buchanan Street, Albany, California 98710 (P.H.Q.)
Transgenic potato (Solanum
tuberosum) plants expressing Arabidopsis phytochrome B were
characterized morphologically and physiologically under white light in
a greenhouse to explore their potential for improved photosynthesis and
higher tuber yields. As expected, overexpression of functional
phytochrome B caused pleiotropic effects such as semidwarfism,
decreased apical dominance, a higher number of smaller but thicker
leaves, and increased pigmentation. Because of increased numbers of
chloroplasts in elongated palisade cells, photosynthesis per leaf area
and in each individual plant increased. In addition, photosynthesis was
less sensitive to photoinactivation under prolonged light stress. The
beginning of senescence was not delayed, but deceleration of
chlorophyll degradation extended the lifetime of photosynthetically
active plants. Both the higher photosynthetic performance and the
longer lifespan of the transgenic plants allowed greater biomass
production, resulting in extended underground organs with increased
tuber yields.
1
This work was supported by the European
Communities BIOTECH program (grant no. BIO2 CT-930400), by a Department
of Energy grant (no. DE-FG03-87ER13742) to P.H.Q., and by a U.S.
Department of Agriculture grant (no. CRIS 5335-21000-010-00D) to P.H.Q.
*
Corresponding author; e-mail cgatz{at}gwdg.de; fax
49-551-397820
Plant Physiol. (1999) 120: 73-82
Copyright Clearance Center: 0032-0889/99/120//10
© 1999 American Society of Plant Physiologists
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