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The Sensitivity of Barley Aleurone Tissue to Gibberellin Is Heterogeneous and May Be Spatially Determined1

Sian Ritchie, Andrew McCubbin, Genevieve Ambrose, Teh-hui Kao, and Simon Gilroy*

Department of Biology, The Pennsylvania State University, 208 Mueller Laboratory, University Park, Pennsylvania 16802 (S.R., G.A., S.G.); and Department of Biochemistry and Molecular Biology, The Pennsylvania State University, 403 Althouse Laboratory, University Park, Pennsylvania 16802 (A.M., T.K.)

In cereals, gibberellin (GA) enhances the synthesis and secretion of hydrolytic enzymes from aleurone cells. These enzymes then mobilize the endosperm storage reserves that fuel germination. The dose-response curve of aleurone protoplasts to GA extends over a range of concentrations from 10-11 to more than 10-6 M. One hypothesis is that subpopulations of cells have different sensitivities to GA, with each cell having a threshold concentration of GA above which it is switched on. The dose-response curve therefore reflects a gradual recruitment of cells to the pool exhibiting a full GA response. Alternatively, all cells may gradually increase their responses as the GA level is increased. In the present study we found that at increasing GA concentrations, increasing numbers of barley (Hordeum vulgare) cells showed the enhanced amylase secretion and vacuolation characteristic of the GA response. We also observed that the region of aleurone tissue closest to the embryo contains the highest proportion of cells activated at the GA concentrations thought to occur naturally in germinating grain. These data indicate that an aleurone layer contains cells of varying sensitivities to GA and that recruitment of these differentially responding pools of cells may explain the broad dose response to GA.

1   This work was supported by grants from the U.S. Department of Agriculture (no. 98-3503-6669 to S.G.) and the National Science Foundation (no. IBN-9603993 to T.K.).
*   Corresponding author; e-mail sxg12{at}psu.edu; fax 1-814-865-9131.

Plant Physiol. (1999) 120: 361-370
Copyright Clearance Center:   0032-0889/99/120//10
© 1999 American Society of Plant Physiologists




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