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Expression of the Granule-Bound Starch Synthase I
(Waxy) Gene from Snapdragon Is Developmentally
and
Circadian Clock Regulated1
Angel Mérida,
José M. Rodríguez-Galán2,
Coral Vincent, and
José M. Romero*
Instituto de Bioquímica Vegetal y Fotosíntesis,
Centro de Investigaciones Científicas Isla de la Cartuja,
C/Americo Vespucio s/n, 41092 Seville, Spain (A.M., J.M.R.-G., J.M.R.); and John Innes Centre, Norwich NR4 7UH, United Kingdom
(C.V.)
The
granule-bound starch synthase I (GBSSI or waxy) enzyme catalyzes one of
the enzymatic steps of starch synthesis. This enzyme is responsible for
the synthesis of amylose and is also involved in building the final
structure of amylopectin. Little is known about expression of GBSSI
genes in tissues other than storage organs, such as seeds, endosperm,
and tuber. We have isolated a gene encoding the GBSSI from snapdragon
(Antirrhinum majus). This gene is present as a single
copy in the snapdragon genome. There is a precise spatial and
developmental regulation of its expression in flowers.
GBSSI expression was observed in all floral whorls at
early developmental stages, but it was restricted to carpel before
anthesis. These results give new insights into the role of starch in
later reproductive events such as seed filling. In leaves the mRNA
level of GBSSI is regulated by an endogenous circadian
clock, indicating that the transition from day to night may be
accompanied by abolition of expression of starch synthesis genes. This
mechanism does not operate in sink tissues such as roots when grown in
the dark.
1
This research was supported by grant no.
PB95-1267 from the Dirección General de Enseñanza Superior,
by Junta de Andalucía group no. CVI-129, and by Spanish-British
Joint Action HB 1995-0042.
2
Present address: Instituto de Recursos Naturales
y Agrobiología. Avda Reina Mercedes no. 10, 41012-Sevilla,
Spain.
*
Corresponding author; e-mail jmromero{at}cica.es; fax
34-54-46-0065.
Plant Physiol. (1999) 120: 401-410
Copyright Clearance Center: 0032-0889/99/120//10
© 1999 American Society of Plant Physiologists
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