Plant Physiol. Illumina
HOME HELP FEEDBACK SUBSCRIPTIONS ARCHIVE SEARCH TABLE OF CONTENTS
QUICK SEARCH:   [advanced]


     

This Article
Right arrow Full Text
Right arrow Full Text (PDF)
Right arrow Alert me when this article is cited
Right arrow Alert me if a correction is posted
Services
Right arrow Email this article to a friend
Right arrow Similar articles in this journal
Right arrow Similar articles in Web of Science
Right arrow Similar articles in PubMed
Right arrow Alert me to new issues of the journal
Right arrow Download to citation manager
Right arrow reprints & permissions
Citing Articles
Right arrow Citing Articles via HighWire
Right arrow Citing Articles via CrossRef
Right arrow Citing Articles via Web of Science (10)
Right arrow Citing Articles via Google Scholar
Google Scholar
Right arrow Articles by Okubara, P. A.
Right arrow Articles by Berry, A. M.
Right arrow Search for Related Content
PubMed
Right arrow PubMed Citation
Right arrow Articles by Okubara, P. A.
Right arrow Articles by Berry, A. M.
Agricola
Right arrow Articles by Okubara, P. A.
Right arrow Articles by Berry, A. M.

Symbiotic Root Nodules of the Actinorhizal Plant Datisca glomerata Express Rubisco Activase mRNA1

Patricia A. Okubara2, Katharina Pawlowski3, Terence M. Murphy, and Alison M. Berry*

Department of Environmental Horticulture (P.A.O., A.M.B.), and Plant Biology Section (T.M.M.), University of California, Davis, California 95616; and University of California, Davis, California 95616Department of Molecular Biology, Wageningen Agricultural University, 6703HA Wageningen, The Netherlands (K.P.)

N2-fixing symbiotic root nodules of the actinorhizal host Datisca glomerata express Dgrca (D. glomerata Rubisco activase) mRNA, a transcript usually associated with photosynthetic organs or tissues. In northern blots a mature, 1700-nucleotide Dgrca mRNA was detected in green plant organs (leaves, flowers, and developing fruits) and in nodules but was not detected in roots. A second message of 3000 nucleotides was observed only in nodules. Both size classes of transcripts were polyadenylated. The larger transcript was 2- to 5-fold more abundant than the mature mRNA; it was hybridized to an intronic probe, indicating that a stable, incompletely spliced transcript was accumulating. Treatment with light on excised nodules did not alter the relative abundance of the two species. In in situ hybridizations the Dgrca message was expressed intensely in the nuclei of infected cells. The Dgrca transcripts also accumulated at lower levels in uninfected cortical cells adjacent to the periderm and the vascular cylinder. mRNA encoding the large subunit of Rubisco (DgrbcL) was abundant in mature infected cells and in the amyloplast-rich sheath of uninfected cortical cells lying between the infected cells and nodule periderm. The proteins Rubisco activase, Rubisco, and the 33-kD O2-evolving complex subunit did not accumulate to detectable levels, indicating that a functional photosynthetic apparatus was not prevalent in nodule tissue. Signals or factors required for the transcription of Dgrca appeared to be present in nodules, but efficient splicing and translation of the message were not observed in Frankia-infected tissue where transcript accumulation was highest.

1   This work was supported by the California Agricultural Experiment Station (project no. 6258 to A.M.B.) and by a Katherine Esau Fellowship from the University of California, Davis, to K.P.
2   Present address: U.S. Department of Agriculture, Agricultural Research Station, Western Regional Research Center, 800 Buchanan Street, Albany, CA 94710.
3   Present address: Albrecht-von-Haller-Institut für Pflanzenwissenschaften, Abteilung Biochemie der Pflanze, Untere Karlspüle 2, 37073 Göttingen, Germany.
*   Corresponding author; e-mail amberry{at}ucdavis.edu; fax 1-530-752-1819.

Plant Physiol. (1999) 120: 411-420
Copyright Clearance Center:   0032-0889/99/120//10
© 1999 American Society of Plant Physiologists




This article has been cited by other articles:


Home page
 Plant Physiol.Home page
J. Bao, S. Lee, C. Chen, X. Zhang, Y. Zhang, S. Liu, T. Clark, J. Wang, M. Cao, H. Yang, et al.
Serial Analysis of Gene Expression Study of a Hybrid Rice Strain (LYP9) and Its Parental Cultivars
Plant Physiology, July 1, 2005; 138(3): 1216 - 1231.
[Abstract] [Full Text] [PDF]

Home page
 Plant Physiol.Home page
A. M. Berry, T. M. Murphy, P. A. Okubara, K. R. Jacobsen, S. M. Swensen, and K. Pawlowski
Novel Expression Pattern of Cytosolic Gln Synthetase in Nitrogen-Fixing Root Nodules of the Actinorhizal Host, Datisca glomerata
Plant Physiology, July 1, 2004; 135(3): 1849 - 1862.
[Abstract] [Full Text] [PDF]

Home page
 Plant Physiol.Home page
P. A. Okubara, N. A. Fujishige, A. M. Hirsch, and A. M. Berry
Dg93, a Nodule-Abundant mRNA of Datisca glomerata with Homology to a Soybean Early Nodulin Gene
Plant Physiology, April 1, 2000; 122(4): 1073 - 1080.
[Abstract] [Full Text]


HOME HELP FEEDBACK SUBSCRIPTIONS ARCHIVE SEARCH TABLE OF CONTENTS
ASPB Publications PLANT PHYSIOLOGY® THE PLANT CELL
Copyright © 1999 by the American Society of Plant Biologists