Plant Physiol. Illumina
HOME HELP FEEDBACK SUBSCRIPTIONS ARCHIVE SEARCH TABLE OF CONTENTS
QUICK SEARCH:   [advanced]


     

This Article
Right arrow Full Text
Right arrow Full Text (PDF)
Right arrow Alert me when this article is cited
Right arrow Alert me if a correction is posted
Services
Right arrow Email this article to a friend
Right arrow Similar articles in this journal
Right arrow Similar articles in Web of Science
Right arrow Similar articles in PubMed
Right arrow Alert me to new issues of the journal
Right arrow Download to citation manager
Right arrow reprints & permissions
Citing Articles
Right arrow Citing Articles via HighWire
Right arrow Citing Articles via CrossRef
Right arrow Citing Articles via Web of Science (14)
Right arrow Citing Articles via Google Scholar
Google Scholar
Right arrow Articles by Inaba, T.
Right arrow Articles by Sasaki, Y.
Right arrow Search for Related Content
PubMed
Right arrow PubMed Citation
Right arrow Articles by Inaba, T.
Right arrow Articles by Sasaki, Y.
Agricola
Right arrow Articles by Inaba, T.
Right arrow Articles by Sasaki, Y.

Identification of a cis-Regulatory Element Involved in Phytochrome Down-Regulated Expression of the Pea Small GTPase Gene pra21

Takehito Inaba, Yukio Nagano, Toshihiro Sakakibara, and Yukiko Sasaki*

Laboratory of Plant Molecular Biology, Graduate School of Bioagricultural Sciences, Nagoya University, Nagoya 464-8601, Japan

The pra2 gene encodes a pea (Pisum sativum) small GTPase belonging to the YPT/rab family, and its expression is down-regulated by light, mediated by phytochrome. We have isolated and characterized a genomic clone of this gene and constructed a fusion DNA of its 5'-upstream region in front of the gene for firefly luciferase. Using this construct in a transient assay, we determined a pra2 cis-regulatory region sufficient to direct the light down-regulation of the luciferase reporter gene. Both 5'- and internal deletion analyses revealed that the 93-bp sequence between -734 and -642 from the transcriptional start site was important for phytochrome down-regulation. Gain-of-function analysis showed that this 93-bp region could confer light down-regulation when fused to the cauliflower mosaic virus 35S promoter. Furthermore, linker-scanning analysis showed that a 12-bp sequence within the 93-bp region mediated phytochrome down-regulation. Gel-retardation analysis showed the presence of a nuclear factor that was specifically bound to the 12-bp sequence in vitro. These results indicate that this element is a cis-regulatory element involved in phytochrome down-regulated expression.

1   This work was supported by grants from the Japanese Ministry of Education, Science, Sports and Culture and from the Japan Society for the Promotion of Science (Research for the Future Program, no. JSPS-RTFT.96L006012). T.I. received Research Fellowships from the Japan Society for the Promotion of Science for Young Scientists.
*   Corresponding author; e-mail sasaki{at}agr.nagoya-u.ac.jp; fax 81-52-789-4296.

Plant Physiol. (1999) 120: 491-500
Copyright Clearance Center:   0032-0889/99/120//10
© 1999 American Society of Plant Physiologists




This article has been cited by other articles:


Home page
 Plant Physiol.Home page
M. E. Hudson and P. H. Quail
Identification of Promoter Motifs Involved in the Network of Phytochrome A-Regulated Gene Expression by Combined Analysis of Genomic Sequence and Microarray Data
Plant Physiology, December 1, 2003; 133(4): 1605 - 1616.
[Abstract] [Full Text]

Home page
 J. Biol. Chem.Home page
T. Inaba, Y. Nagano, T. Nagasaki, and Y. Sasaki
Distinct Localization of Two Closely Related Ypt3/Rab11 Proteins on the Trafficking Pathway in Higher Plants
J. Biol. Chem., March 8, 2002; 277(11): 9183 - 9188.
[Abstract] [Full Text] [PDF]

Home page
 Nucleic Acids ResHome page
Y. Nagano, H. Furuhashi, T. Inaba, and Y. Sasaki
A novel class of plant-specific zinc-dependent DNA-binding protein that binds to A/T-rich DNA sequences
Nucleic Acids Res., October 15, 2001; 29(20): 4097 - 4105.
[Abstract] [Full Text] [PDF]

Home page
 Plant Cell PhysiolHome page
S. Makino, A. Matsushika, M. Kojima, Y. Oda, and T. Mizuno
Light Response of the Circadian Waves of the APRR1/TOC1 Quintet: When Does the Quintet Start Singing Rhythmically in Arabidopsis?
Plant Cell Physiol., March 1, 2001; 42(3): 334 - 339.
[Abstract] [Full Text] [PDF]

Home page
 J. Biol. Chem.Home page
T. Inaba, Y. Nagano, J. B. Reid, and Y. Sasaki
DE1, a 12-Base Pair cis-Regulatory Element Sufficient to Confer Dark-inducible and Light Down-regulated Expression to a Minimal Promoter in Pea
J. Biol. Chem., June 23, 2000; 275(26): 19723 - 19727.
[Abstract] [Full Text] [PDF]

Home page
 J. Biol. Chem.Home page
Y. Nagano, T. Inaba, H. Furuhashi, and Y. Sasaki
Trihelix DNA-binding Protein with Specificities for Two Distinct cis-Elements. BOTH IMPORTANT FOR LIGHT DOWN-REGULATED AND DARK-INDUCIBLE GENE EXPRESSION IN HIGHER PLANTS
J. Biol. Chem., June 15, 2001; 276(25): 22238 - 22243.
[Abstract] [Full Text] [PDF]


HOME HELP FEEDBACK SUBSCRIPTIONS ARCHIVE SEARCH TABLE OF CONTENTS
ASPB Publications PLANT PHYSIOLOGY® THE PLANT CELL
Copyright © 1999 by the American Society of Plant Biologists