Plant Physiol.
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Influence of White Clover Mosaic Potexvirus Infection on the Endogenous Cytokinin Content of Bean

Sean Francis Clarke1, Marian Jane McKenzie2, David John Burritt, Paul Leslie Guy, and Paula Elizabeth Jameson*

Department of Botany, University of Otago, P.O. Box 56, Dunedin, New Zealand (S.F.C., D.J.B., P.L.G.); and Institute of Molecular BioSciences, College of Sciences, Massey University, Private Bag 11 222, Palmerston North, New Zealand (M.J.M., P.E.J.)

The cytokinin content in the primary leaves of bean (Phaseolus vulgaris) was monitored for 10 d after inoculation with white clover mosaic potexvirus. The cytokinins were isolated, purified, separated by high-performance liquid chromatography, and quantified by radioimmunoassay. The cytokinins detected at the time of inoculation (d 0) were: (a) the free bases, zeatin (Z), dihydrozeatin (DZ), and isopentenyladenine; (b) the riboside, DZ riboside (DZR); (c) the O-glucosides of DZ, DZR, and Z riboside; (d) the nucleotides, Z riboside-5'-monophosphate and isopentenyladenosine-5'-monophosphate; and (e) trace amounts of Z-9-glucoside and DZ-9-glucoside. During the 10 d after inoculation with white clover mosaic potexvirus, marked quantitative changes in this cytokinin profile were observed. The concentration of the free bases and DZR decreased, accompanied by an increase in the 9-glucosides and the nucleotides. Virus titer increased rapidly 3 d after inoculation, attaining a maximum level at d 5. This increase coincided with the increases in the 9-glucosides and the nucleotides. We propose that the decline in the cytokinin free bases and riboside may allow the increase of virus titer in bean and lead to the senescence of infected leaves.

1   Present address: National Institute of Biological Standards and Control, Blanche Lane, South Mimms, Potters Bar EN6 3QG, UK.
2   Present address: The New Zealand Institute for Crop & Food Research Limited, Private Bag 11600, Palmerston North, New Zealand.
*   Corresponding author; e-mail P.E.Jameson{at}massey.ac.nz; fax 64-6-3505694.

Plant Physiol. (1999) 120: 547-552
Copyright Clearance Center:   0032-0889/99/120//06
© 1999 American Society of Plant Physiologists




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[Abstract] [Full Text] [PDF]


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