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Aldehyde Oxidase and Xanthine Dehydrogenase in a
flacca Tomato Mutant with Deficient Abscisic Acid and
Wilty Phenotype1
Moshe Sagi*,
Robert Fluhr, and
S. Herman Lips
Biostress Research Laboratory, Department of Life Science, Faculty
of Natural Sciences, Ben-Gurion University of the Negev, Sede Boqer
84990, Israel (M.S., S.H.L.); and Department of Plant Science,
Weizman Institute of Science, Rechovot, Israel (R.F.)
The flacca tomato
(Lycopersicon esculentum) mutant displays a wilty
phenotype as a result of abscisic acid (ABA) deficiency. The Mo
cofactor (MoCo)-containing aldehyde oxidases (AO; EC 1.2.3.1) are
thought to play a role in the final oxidation step required for ABA
biosynthesis. AO and related MoCo-containing enzymes xanthine dehydrogenase (XDH; EC 1.2.1.37) and nitrate reductase (EC 1.6.6.1) were examined in extracts of the flacca tomato genotype
and of wild-type (WT) roots and shoots. The levels of MoCo were found to be similar in both genotypes. No significant XDH or AO
(MoCo-containing hydroxylases) activities were detected in
flacca leaves; however, the mutant exhibited
considerable MoCo-containing hydroxylase activity in the roots, which
contained notable amounts of ABA. Native western blots probed with an
antibody to MoCo-containing hydroxylases revealed substantial, albeit
reduced, levels of cross-reactive protein in the flacca
mutant shoots and roots. The ABA xylem-loading rate was significantly
lower than that in the WT, indicating that the flacca is
also defective in ABA transport to the shoot. Significantly, in vitro
sulfurylation with Na2S reactivated preexisting XDH and AO
proteins in extracts from flacca, particularly
from the shoots, and superinduced the basal-level activity in
the WT extracts. The results indicate that in flacca,
MoCo-sulfurylase activity is impaired in a tissue-dependent manner.
1
This work was supported by the U.S. Agency for
International Development/Cooperative Development Research
(project nos. C12-157 and CA15-024), by the Fohs Foundation, Israel
Charitable Association, and by the Jewish National Foundation
(the Ramat Negev Research and Development Project).
*
Corresponding author; e-mail gizi{at}bgumail.bgu.ac.il; fax
972-7-6596752.
Plant Physiol. (1999) 120: 571-578
Copyright Clearance Center: 0032-0889/99/120//08
© 1999 American Society of Plant Physiologists
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