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Chlamydomonas reinhardtii Mutants Abnormal in Their Responses to Phosphorus Deprivation1

Kosuke Shimogawara, Dennis D. Wykoff, Hideaki Usuda, and Arthur R. Grossman*

Laboratory of Chemistry, Teikyo University School of Medicine, Hachioji, Tokyo, 192-0395 Japan (K.S., H.U.); and Department of Plant Biology, Carnegie Institution of Washington, 260 Panama Street, Stanford, California 94305 (D.D.W., A.R.G.)

P-starved plants scavenge inorganic phosphate (Pi) by developing elevated rates of Pi uptake, synthesizing extracellular phosphatases, and secreting organic acids. To elucidate mechanisms controlling these acclimation responses in photosynthetic organisms, we characterized the responses of the green alga Chlamydomonas reinhardtii to P starvation and developed screens for isolating mutants (designated psr [phosphorus-stress response]) abnormal in their responses to environmental levels of Pi. The psr1-1 mutant was identified in a selection for cells that survived exposure to high concentrations of radioactive Pi. psr1-2 and psr2 were isolated as strains with aberrant levels of extracellular phosphatase activity during P-deficient or nutrient-replete growth. The psr1-1 and psr1-2 mutants were phenotypically similar, and the lesions in these strains were recessive and allelic. They exhibited no increase in extracellular phosphatase activity or Pi uptake upon starvation. Furthermore, when placed in medium devoid of P, the psr1 strains lost photosynthetic O2 evolution and stopped growing more rapidly than wild-type cells; they may not be as efficient as wild-type cells at scavenging/accessing P stores. In contrast, psr2 showed elevated extracellular phosphatase activity during growth in nutrient-replete medium, and the mutation was dominant. The mutant phenotypes and the roles of Psr1 and Psr2 in P-limitation responses are discussed.

1   This work was supported by the Japan-U.S. Cooperative Science Program from Japan Society of Plant Physiologists (JSPS) and the National Science Foundation (no. INT 9513 133 to H.U. and A.R.G.), the Research for the Future Program (no. JSPS-RFTF97R16001) from JSPS (to H.U.), the Asahi Glass Foundation (to K.S.), the Ministry of Education, Science, Sports and Culture, Japan (to K.S.), and the U.S. Department of Agriculture (grant no. 9302076 to A.R.G.). This is Carnegie Institution of Washington publication no. 1412.
*   Corresponding author; e-mail arthur{at}andrew2.stanford.edu; fax 1-650-325-6857.

Plant Physiol. (1999) 120: 685-694
Copyright Clearance Center:   0032-0889/99/120//10
© 1999 American Society of Plant Physiologists




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