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Simultaneous Expression of NAD-Dependent Isocitrate Dehydrogenase
and Other Krebs Cycle Genes after Nitrate Resupply to Short-Term
Nitrogen-Starved Tobacco
Muriel Lancien1,
Sylvie Ferrario-Méry,
Yvette Roux,
Evelyne Bismuth,
Céline Masclaux,
Bertrand Hirel,
Pierre Gadal, and
Michael Hodges*
Institut de Biotechnologie des Plantes, Unité Mixte de la
Recherche 8618 Centre National de la Recherche Scientifique,
Bât. 630, Université Paris Sud-XI, 91405 Orsay
cedex, France (M.L., E.B., P.G., M.H.); and Laboratoire du
Métabolisme et de la Nutrition des Plantes, Institut National de
la Recherche Agronomique, Route de Saint-Cyr, 78026 Versailles cedex,
France (S.F.-M., Y.R., C.M., B.H.)
Mitochondrial NAD-dependent (IDH) and
cytosolic NADP-dependent isocitrate dehydrogenases have been considered
as candidates for the production of 2-oxoglutarate required by the
glutamine synthetase/glutamate synthase cycle. The increase in IDH
transcripts in leaf and root tissues, induced by nitrate or
NH4+ resupply to short-term N-starved tobacco
(Nicotiana tabacum) plants, suggested that this enzyme
could play such a role. The leaf and root steady-state mRNA levels of
citrate synthase, acotinase, IDH, and glutamine synthetase were found
to respond similarly to nitrate, whereas those for cytosolic
NADP-dependent isocitrate dehydrogenase and fumarase responded
differently. This apparent coordination occurred only at the mRNA
level, since activity and protein levels of certain corresponding
enzymes were not altered. Roots and leaves were not affected to the
same extent either by N starvation or nitrate addition, the roots
showing smaller changes in N metabolite levels. After nitrate resupply,
these organs showed different response kinetics with respect to mRNA
and N metabolite levels, suggesting that under such conditions nitrate
assimilation was preferentially carried out in the roots. The
differential effects appeared to reflect the C/N status after N
starvation, the response kinetics being associated with the nitrate
assimilatory capacity of each organ, signaled either by nitrate status
or by metabolite(s) associated with its metabolism.
1
Present address: Laboratory of Molecular
Virology, Institute of Molecular Agrobiology, 1 Research Link, The
National University of Singapore, Singapore 117604.
*
Corresponding author; e-mail hodges{at}sidonie.ibp.u-psud.fr; fax
33-1-69-33-64-23.
Plant Physiol. (1999) 120: 717-726
Copyright Clearance Center: 0032-0889/99/120//10
© 1999 American Society of Plant Physiologists
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