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Xanthophyll Cycle Pigment Localization and Dynamics during Exposure to Low Temperatures and Light Stress in Vinca major1

Amy S. Verhoeven2, *, William W. Adams III, Barbara Demmig-Adams, Roberta Croce, and Roberto Bassi

Department of Environmental, Population, and Organismic Biology, University of Colorado, Boulder, Colorado 80309-0334 (A.S.V., W.W.A., B.D.-A.); and Universita di Verona, Facolta di Scienze Matematiche, Fisiche e Naturali, Biotecnologie Vegetali, Strada Le Grazie, 37134 Verona, Italy (R.C., R.B.)

The distribution of xanthophyll cycle pigments (violaxanthin plus antheraxanthin plus zeaxanthin [VAZ]) among photosynthetic pigment-protein complexes was examined in Vinca major before, during, and subsequent to a photoinhibitory treatment at low temperature. Four pigment-protein complexes were isolated: the core of photosystem (PS) II, the major light-harvesting complex (LHC) protein of PSII (LHCII), the minor light-harvesting proteins (CPs) of PSII (CP29, CP26, and CP24), and PSI with its LHC proteins (PSI-LHCI). In isolated thylakoids 80% of VAZ was bound to protein independently of the de-epoxidation state and was found in all complexes. Plants grown outside in natural sunlight had higher levels of VAZ (expressed per chlorophyll), compared with plants grown in low light in the laboratory, and the additional VAZ was mainly bound to the major LHCII complex, apparently in an acid-labile site. The extent of de-epoxidation of VAZ in high light and the rate of reconversion of Z plus A to V following 2.5 h of recovery were greatest in the free-pigment fraction and varied among the pigment-protein complexes. Photoinhibition caused increases in VAZ, particularly in low-light-acclimated leaves. The data suggest that the photoinhibitory treatment caused an enrichment in VAZ bound to the minor CPs caused by de novo synthesis of the pigments and/or a redistribution of VAZ from the major LHCII complex.

1   This work was supported by the U.S. National Science Foundation (award no. IBN-9631064 to W.W.A. and B.D.-A.), a fellowship from the David and Lucile Packard Foundation to B.D.-A., grants from the Ministry of University and Scientific Research, and the Biotechnology Project of the Italian National Research Council to R.B.
2   Present address: University of St. Thomas, Department of Biology, 2115 Summit Avenue, St. Paul, MN 55105-1096.
*   Corresponding author; e-mail verhoeve{at}hawaii.edu; fax (Hawaii) 1-808-956-3542; fax (Minnesota) 1-651-962-5209.

Plant Physiol. (1999) 120: 727-738
Copyright Clearance Center:   0032-0889/99/120//12
© 1999 American Society of Plant Physiologists




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