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Regions of the Pea Lhcb1*4 Promoter Necessary for Blue-Light Regulation in Transgenic Arabidopsis1

Kevin M. Folta and Lon S. Kaufman*

Laboratory for Molecular Biology, Department of Biological Sciences, University of Illinois at Chicago, Chicago, Illinois 60607

Pea (Pisum sativum) and Arabidopsis contain similar, if not identical, blue-light (BL)-responsive systems that alter expression of specific members of the Lhcb (light-harvesting chlorophyll-binding) gene family. In both plants a single, short pulse of low-fluence BL (threshold = 10-1 µmol m-2) causes an increase in the rate of transcription from specific members of the Lhcb gene family in etiolated seedlings. Constructs of the BL-regulated pea Lhcb1*4 promoter (PsLhcb1*4) were created, which altered sequences previously implicated in light responses, deleted the 5'-promoter sequence, or removed the 5'-untranslated region. These constructs were tested for BL induction in transgenic Arabidopsis. The PsLhcb1*4 promoter deletions to -150 bp maintained normal fluence response, time course, and reciprocity characteristics. The 5'- untranslated region contained enhancer elements, but was not necessary for BL induction. The -95 to +2 promoter was capable of responding to BL, whereas sequences from -50 were not. Promoters that lack conserved light-regulatory elements or sequences directly implicated in phytochrome and circadian responses retained BL activity, suggesting that the low-fluence BL response utilizes regions of the promoter independent of those that modulate the phytochrome and circadian responses.

1   This work was supported in part by U.S. Department of Agriculture grant no. 9701418.
*   Corresponding author; e-mail lkaufman{at}uic.edu; fax 1-312-413-2691.

Plant Physiol. (1999) 120: 747-756
Copyright Clearance Center:   0032-0889/99/120//10
© 1999 American Society of Plant Physiologists




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