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Periplasmic Carbonic Anhydrase Structural Gene (Cah1) Mutant in Chlamydomonas reinhardtii 1

Kyujung Van and Martin H. Spalding*

Interdepartmental Plant Physiology Major and Department of Botany, 353 Bessey Hall, Iowa State University, Ames, Iowa 50011

To survive in various conditions of CO2 availability, Chlamydomonas reinhardtii shows adaptive changes, such as induction of a CO2-concentrating mechanism, changes in cell organization, and induction of several genes, including a periplasmic carbonic anhydrase (pCA1) encoded by Cah1. Among a collection of insertionally generated mutants, a mutant has been isolated that showed no pCA1 protein and no Cah1 mRNA. This mutant strain, designated cah1-1, has been confirmed to have a disruption in the Cah1 gene caused by a single Arg7 insert. The most interesting feature of cah1-1 is its lack of any significant growth phenotype. There is no major difference in growth or photosynthesis between the wild type and cah1-1 over a pH range from 5.0 to 9.0 even though this mutant apparently lacks Cah1 expression in air. Although the presence of pCA1 apparently gives some minor benefit at very low CO2 concentrations, the characteristics of this Cah1 null mutant demonstrate that pCA1 is not essential for function of the CO2-concentrating mechanism or for growth of C. reinhardtii at limiting CO2 concentrations.

1   This work was supported by the U.S. Department of Agriculture National Research Initiative (grant no. 97-35100-4210 to M.H.S.). This is journal paper no. J-18217 of project no. 3479 of the Iowa Agriculture and Home Economics Experiment Station, Ames, and was supported by the Hatch Act and State of Iowa funds.
*   Corresponding author; e-mail mspaldin{at}iastate.edu; fax 1-515-294-1377.

Plant Physiol. (1999) 120: 757-764
Copyright Clearance Center:   0032-0889/99/120//08
© 1999 American Society of Plant Physiologists




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