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Heavy-Metal Regulation of Thioredoxin Gene Expression in Chlamydomonas reinhardtii

Stéphane Lemaire*, Eliane Keryer, Mariana Stein, Isabelle Schepens, Emmanuelle Issakidis-Bourguet, Catherine Gérard-Hirne, Myroslawa Miginiac-Maslow, and Jean-Pierre Jacquot

Institut de Biotechnologie des Plantes, Únite Mixte de Recherche 8618, Centre National de la Recherche Scientifique, Université Paris-Sud, Bâtiment 630, 91405 Orsay cedex, France (S.L., E.K., M.S., I.S., E.I.-B., C.G.-H., M.M.-M.); and Laboratoire de Biologie Forestière, Université de Nancy I, BP239, 54506 Vandoeuvre, France (J.P.J.)

Heavy metals are highly toxic compounds for cells. In this report we demonstrate that the expression of Chlamydomonas reinhardtii thioredoxins (TRX) m and h is induced by heavy metals. Upon exposure of the cells to Cd and Hg, a strong accumulation of both messengers was observed. Western-blot experiments revealed that among these two TRXs, only TRX h polypeptides accumulated in response to the toxic cations. A biochemical analysis indicated that heavy metals inhibit TRX activity, presumably by binding at the level of their active site. Sequence analysis of the C. reinhardtii TRX h promoter revealed the presence of cis-acting elements related to cadmium induction. The origins and purposes of this regulation are discussed. Our data suggest, for the first time to our knowledge, a possible implication of TRXs in defense mechanisms against heavy metals.

*   Corresponding author; e-mail slemaire{at}ibp.u-psud.fr; fax 33-1-69-33-64-23.

Plant Physiol. (1999) 120: 773-778
Copyright Clearance Center:   0032-0889/99/120//06
© 1999 American Society of Plant Physiologists




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