Plant Physiol. Tips for Better Browsing
HOME HELP FEEDBACK SUBSCRIPTIONS ARCHIVE SEARCH TABLE OF CONTENTS
QUICK SEARCH:   [advanced]


     

This Article
Right arrow Full Text
Right arrow Full Text (PDF)
Right arrow Alert me when this article is cited
Right arrow Alert me if a correction is posted
Services
Right arrow Email this article to a friend
Right arrow Similar articles in this journal
Right arrow Similar articles in Web of Science
Right arrow Similar articles in PubMed
Right arrow Alert me to new issues of the journal
Right arrow Download to citation manager
Right arrow reprints & permissions
Citing Articles
Right arrow Citing Articles via HighWire
Right arrow Citing Articles via CrossRef
Right arrow Citing Articles via Web of Science (24)
Right arrow Citing Articles via Google Scholar
Google Scholar
Right arrow Articles by Lemaire, S.
Right arrow Articles by Jacquot, J.-P.
Right arrow Search for Related Content
PubMed
Right arrow PubMed Citation
Right arrow Articles by Lemaire, S.
Right arrow Articles by Jacquot, J.-P.
Agricola
Right arrow Articles by Lemaire, S.
Right arrow Articles by Jacquot, J.-P.

Heavy-Metal Regulation of Thioredoxin Gene Expression in Chlamydomonas reinhardtii

Stéphane Lemaire*, Eliane Keryer, Mariana Stein, Isabelle Schepens, Emmanuelle Issakidis-Bourguet, Catherine Gérard-Hirne, Myroslawa Miginiac-Maslow, and Jean-Pierre Jacquot

Institut de Biotechnologie des Plantes, Únite Mixte de Recherche 8618, Centre National de la Recherche Scientifique, Université Paris-Sud, Bâtiment 630, 91405 Orsay cedex, France (S.L., E.K., M.S., I.S., E.I.-B., C.G.-H., M.M.-M.); and Laboratoire de Biologie Forestière, Université de Nancy I, BP239, 54506 Vandoeuvre, France (J.P.J.)

Heavy metals are highly toxic compounds for cells. In this report we demonstrate that the expression of Chlamydomonas reinhardtii thioredoxins (TRX) m and h is induced by heavy metals. Upon exposure of the cells to Cd and Hg, a strong accumulation of both messengers was observed. Western-blot experiments revealed that among these two TRXs, only TRX h polypeptides accumulated in response to the toxic cations. A biochemical analysis indicated that heavy metals inhibit TRX activity, presumably by binding at the level of their active site. Sequence analysis of the C. reinhardtii TRX h promoter revealed the presence of cis-acting elements related to cadmium induction. The origins and purposes of this regulation are discussed. Our data suggest, for the first time to our knowledge, a possible implication of TRXs in defense mechanisms against heavy metals.

*   Corresponding author; e-mail slemaire{at}ibp.u-psud.fr; fax 33-1-69-33-64-23.

Plant Physiol. (1999) 120: 773-778
Copyright Clearance Center:   0032-0889/99/120//06
© 1999 American Society of Plant Physiologists




This article has been cited by other articles:


Home page
 Mol PlantHome page
M. E. Perez-Perez, E. Martin-Figueroa, and F. J. Florencio
Photosynthetic Regulation of the Cyanobacterium Synechocystis sp. PCC 6803 Thioredoxin System and Functional Analysis of TrxB (Trx x) and TrxQ (Trx y) Thioredoxins
Mol Plant, March 1, 2009; 2(2): 270 - 283.
[Abstract] [Full Text] [PDF]

Home page
 J Exp BotHome page
J. A. Traverso, F. Vignols, R. Cazalis, A. J. Serrato, P. Pulido, M. Sahrawy, Y. Meyer, F. J. Cejudo, and A. Chueca
Immunocytochemical localization of Pisum sativum TRXs f and m in non-photosynthetic tissues
J. Exp. Bot., April 1, 2008; 59(6): 1267 - 1277.
[Abstract] [Full Text] [PDF]

Home page
 Eukaryot CellHome page
N. Sarkar, S. Lemaire, D. Wu-Scharf, E. Issakidis-Bourguet, and H. Cerutti
Functional Specialization of Chlamydomonas reinhardtii Cytosolic Thioredoxin h1 in the Response to Alkylation-Induced DNA Damage
Eukaryot. Cell, February 1, 2005; 4(2): 262 - 273.
[Abstract] [Full Text] [PDF]

Home page
 Plant Physiol.Home page
C. Laloi, D. Mestres-Ortega, Y. Marco, Y. Meyer, and J.-P. Reichheld
The Arabidopsis Cytosolic Thioredoxin h5 Gene Induction by Oxidative Stress and Its W-Box-Mediated Response to Pathogen Elicitor
Plant Physiology, March 1, 2004; 134(3): 1006 - 1016.
[Abstract] [Full Text] [PDF]


HOME HELP FEEDBACK SUBSCRIPTIONS ARCHIVE SEARCH TABLE OF CONTENTS
ASPB Publications PLANT PHYSIOLOGY® THE PLANT CELL
Copyright © 1999 by the American Society of Plant Biologists