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Expansins Are Conserved in Conifers and Expressed in Hypocotyls
in Response to Exogenous Auxin1
Keith W. Hutchison*,
Patricia B. Singer,
Stephanie McInnis,
Carmen Diaz-Sala2, and
Michael S. Greenwood
Department of Biochemistry, Microbiology, and Molecular Biology
(K.W.H., P.B.S., S.M., C.D.-S.) and Department of Forest Ecosystem
Science (M.S.G.), The University of Maine, Orono, Maine 04469
Differential
display reverse transcription-polymerase chain reaction was used to
detect the induction of gene expression during adventitious root
formation in loblolly pine (Pinus taeda) after treatment
with the exogenous auxin indole-3-butyric acid. A BLAST search of the
GenBank database using one of the clones obtained revealed very strong
similarity to the  -expansin gene family in angiosperms. A
near-full-length loblolly pine -expansin sequence was obtained using
5 - and 3-rapid amplification of cDNA end cloning, and the deduced
amino acid sequence was highly conserved relative to those of
angiosperm expansins. Northern analysis indicates that -expansin
mRNA expression increases 50- to 100-fold in the base of hypocotyl stem
cuttings from loblolly pine seedlings in response to indole-3-butyric
acid, with peak expression occurring 24 to 48 h after induction.
1
This work was supported by a grant from the
Maine Agriculture and Forestry Experiment Station (K.W.H.) and the
North Carolina State Loblolly and Slash Pine Rooted Cutting Project
(M.S.G.). This is Maine Agriculture and Forestry Experiment Station
publication no. 2321.
2
Present address: Department of Biologica
Vegetal, Universidad Alcala de Henares, 28871 Alcala de Henares,
Madrid, Spain.
*
Corresponding author; e-mail keithh{at}maine.edu; fax
1-207-581-2801.
Plant Physiol. (1999) 120: 827-832
Copyright Clearance Center: 0032-0889/99/120//06
© 1999 American Society of Plant Physiologists
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