Plant Physiol.
HOME HELP FEEDBACK SUBSCRIPTIONS ARCHIVE SEARCH TABLE OF CONTENTS
QUICK SEARCH:   [advanced]


     

This Article
Right arrow Full Text
Right arrow Full Text (PDF)
Right arrow Alert me when this article is cited
Right arrow Alert me if a correction is posted
Services
Right arrow Email this article to a friend
Right arrow Similar articles in this journal
Right arrow Similar articles in ISI Web of Science
Right arrow Similar articles in PubMed
Right arrow Alert me to new issues of the journal
Right arrow Download to citation manager
Right arrow reprints & permissions
Citing Articles
Right arrow Citing Articles via HighWire
Right arrow Citing Articles via CrossRef
Right arrow Citing Articles via ISI Web of Science (56)
Right arrow Citing Articles via Google Scholar
Google Scholar
Right arrow Articles by Gordon, A. J.
Right arrow Articles by Komina, O.
Right arrow Search for Related Content
PubMed
Right arrow PubMed Citation
Right arrow Articles by Gordon, A. J.
Right arrow Articles by Komina, O.
Agricola
Right arrow Articles by Gordon, A. J.
Right arrow Articles by Komina, O.

Sucrose Synthase in Legume Nodules Is Essential for Nitrogen Fixation1

Anthony J. Gordon*, Frank R. Minchin, Caron L. James, and Olga Komina2

Department of Environmental Biology, Institute of Grassland and Environmental Research, Plas Gogerddan, Aberystwyth, Ceridigion SY23 3EB, United Kingdom

The role of sucrose synthase (SS) in the fixation of N was examined in the rug4 mutant of pea (Pisum sativum L.) plants in which SS activity was severely reduced. When dependent on nodules for their N supply, the mutant plants were not viable and appeared to be incapable of effective N fixation, although nodule formation was essentially normal. In fact, N and C resources invested in nodules were much greater in mutant plants than in the wild-type (WT) plants. Low SS activity in nodules (present at only 10% of WT levels) resulted in lower amounts of total soluble protein and leghemoglobin and lower activities of several enzymes compared with WT nodules. Alkaline invertase activity was not increased to compensate for reduced SS activity. Leghemoglobin was present at less than 20% of WT values, so O2 flux may have been compromised. The two components of nitrogenase were present at normal levels in mutant nodules. However, only a trace of nitrogenase activity was detected in intact plants and none was found in isolated bacteroids. The results are discussed in relation to the role of SS in the provision of C substrates for N fixation and in the development of functional nodules.

1   The Institute of Grassland and Environmental Research is funded by the Biotechnology and Biological Sciences Research Council of the United Kingdom. O.K. was supported by the Royal Society.
2   Present address: A.N. Bach Institute of Biochemistry, Leninsky Pr 33, Moscow 117071, Russia.
*   Corresponding author; e-mail tony.gordon{at}bbsrc.ac.uk; fax 44-9-70-828-357.

Plant Physiol. (1999) 120: 867-878
Copyright Clearance Center:   0032-0889/99/120//12
© 1999 American Society of Plant Physiologists




This article has been cited by other articles:


Home page
 Plant Physiol.Home page
F. J. Redondo, T. C. de la Pena, C. N. Morcillo, M. M. Lucas, and J. J. Pueyo
Overexpression of Flavodoxin in Bacteroids Induces Changes in Antioxidant Metabolism Leading to Delayed Senescence and Starch Accumulation in Alfalfa Root Nodules
Plant Physiology, February 1, 2009; 149(2): 1166 - 1178.
[Abstract] [Full Text] [PDF]

Home page
 J Exp BotHome page
S. Wienkoop, E. Larrainzar, M. Glinski, E. M. Gonzalez, C. Arrese-Igor, and W. Weckwerth
Absolute quantification of Medicago truncatula sucrose synthase isoforms and N-metabolism enzymes in symbiotic root nodules and the detection of novel nodule phosphoproteins by mass spectrometry
J. Exp. Bot., September 1, 2008; 59(12): 3307 - 3315.
[Abstract] [Full Text] [PDF]

Home page
 Plant Physiol.Home page
P. D.R. van Heerden, G. Kiddle, T. K. Pellny, P. W. Mokwala, A. Jordaan, A. J. Strauss, M. de Beer, U. Schluter, K. J. Kunert, and C. H. Foyer
Regulation of Respiration and the Oxygen Diffusion Barrier in Soybean Protect Symbiotic Nitrogen Fixation from Chilling-Induced Inhibition and Shoots from Premature Senescence
Plant Physiology, September 1, 2008; 148(1): 316 - 327.
[Abstract] [Full Text] [PDF]

Home page
 J Exp BotHome page
S. Silvente, P. M. Reddy, S. Khandual, L. Blanco, X. Alvarado-Affantranger, F. Sanchez, and M. Lara-Flores
Evidence for sugar signalling in the regulation of asparagine synthetase gene expressed in Phaseolus vulgaris roots and nodules
J. Exp. Bot., April 11, 2008; (2008) ern034v1.
[Abstract] [Full Text] [PDF]

Home page
 Plant Physiol.Home page
M. C. Baier, A. Barsch, H. Kuster, and N. Hohnjec
Antisense Repression of the Medicago truncatula Nodule-Enhanced Sucrose Synthase Leads to a Handicapped Nitrogen Fixation Mirrored by Specific Alterations in the Symbiotic Transcriptome and Metabolome
Plant Physiology, December 1, 2007; 145(4): 1600 - 1618.
[Abstract] [Full Text] [PDF]

Home page
 Plant Physiol.Home page
R. Ladrera, D. Marino, E. Larrainzar, E. M. Gonzalez, and C. Arrese-Igor
Reduced Carbon Availability to Bacteroids and Elevated Ureides in Nodules, But Not in Shoots, Are Involved in the Nitrogen Fixation Response to Early Drought in Soybean
Plant Physiology, October 1, 2007; 145(2): 539 - 546.
[Abstract] [Full Text] [PDF]

Home page
 Plant Physiol.Home page
J. White, J. Prell, E. K. James, and P. Poole
Nutrient Sharing between Symbionts
Plant Physiology, June 1, 2007; 144(2): 604 - 614.
[Full Text] [PDF]

Home page
 Plant Physiol.Home page
I. Horst, T. Welham, S. Kelly, T. Kaneko, S. Sato, S. Tabata, M. Parniske, and T. L. Wang
TILLING Mutants of Lotus japonicus Reveal That Nitrogen Assimilation and Fixation Can Occur in the Absence of Nodule-Enhanced Sucrose Synthase
Plant Physiology, June 1, 2007; 144(2): 806 - 820.
[Abstract] [Full Text] [PDF]

Home page
 Plant Physiol.Home page
L. Naya, R. Ladrera, J. Ramos, E. M. Gonzalez, C. Arrese-Igor, F. R. Minchin, and M. Becana
The Response of Carbon Metabolism and Antioxidant Defenses of Alfalfa Nodules to Drought Stress and to the Subsequent Recovery of Plants
Plant Physiology, June 1, 2007; 144(2): 1104 - 1114.
[Abstract] [Full Text] [PDF]

Home page
 Plant Physiol.Home page
A. C. Cumino, C. Marcozzi, R. Barreiro, and G. L. Salerno
Carbon Cycling in Anabaena sp. PCC 7120. Sucrose Synthesis in the Heterocysts and Possible Role in Nitrogen Fixation
Plant Physiology, March 1, 2007; 143(3): 1385 - 1397.
[Abstract] [Full Text] [PDF]

Home page
 Plant Physiol.Home page
C. G. Starker, A. L. Parra-Colmenares, L. Smith, R. M. Mitra, and S. R. Long
Nitrogen Fixation Mutants of Medicago truncatula Fail to Support Plant and Bacterial Symbiotic Gene Expression
Plant Physiology, February 1, 2006; 140(2): 671 - 680.
[Abstract] [Full Text] [PDF]

Home page
 J Exp BotHome page
L. Galvez, E. M. Gonzalez, and C. Arrese-Igor
Evidence for carbon flux shortage and strong carbon/nitrogen interactions in pea nodules at early stages of water stress
J. Exp. Bot., September 1, 2005; 56(419): 2551 - 2561.
[Abstract] [Full Text] [PDF]

Home page
 Plant CellHome page
L. Krusell, K. Krause, T. Ott, G. Desbrosses, U. Kramer, S. Sato, Y. Nakamura, S. Tabata, E. K. James, N. Sandal, et al.
The Sulfate Transporter SST1 Is Crucial for Symbiotic Nitrogen Fixation in Lotus japonicus Root Nodules
PLANT CELL, May 1, 2005; 17(5): 1625 - 1636.
[Abstract] [Full Text] [PDF]

Home page
 Plant Physiol.Home page
G. G. Desbrosses, J. Kopka, and M. K. Udvardi
Lotus japonicus Metabolic Profiling. Development of Gas Chromatography-Mass Spectrometry Resources for the Study of Plant-Microbe Interactions
Plant Physiology, April 1, 2005; 137(4): 1302 - 1318.
[Abstract] [Full Text] [PDF]

Home page
 J Exp BotHome page
E. Etxeberria and P. Gonzalez
Evidence for a tonoplast-associated form of sucrose synthase and its potential involvement in sucrose mobilization from the vacuole
J. Exp. Bot., May 1, 2003; 54(386): 1407 - 1414.
[Abstract] [Full Text] [PDF]

Home page
 J Exp BotHome page
S. Silvente, A. Camas, and M. Lara
Heterogeneity of sucrose synthase genes in bean (Phaseolus vulgaris L.): evidence for a nodule-enhanced sucrose synthase gene
J. Exp. Bot., February 1, 2003; 54(383): 749 - 755.
[Abstract] [Full Text] [PDF]

Home page
 J Exp BotHome page
E. Goulas, F. Le Dily, J.-C. Simon, and A. Ourry
Morphological pattern of development affects the contribution of nitrogen reserves to regrowth of defoliated white clover (Trifolium repens L.)
J. Exp. Bot., September 1, 2002; 53(376): 1941 - 1948.
[Abstract] [Full Text] [PDF]

Home page
 J Exp BotHome page
A.J. Gordon, L. Skot, C.L. James, and F.R. Minchin
Short-term metabolic responses of soybean root nodules to nitrate
J. Exp. Bot., March 1, 2002; 53(368): 423 - 428.
[Abstract] [Full Text] [PDF]


HOME HELP FEEDBACK SUBSCRIPTIONS ARCHIVE SEARCH TABLE OF CONTENTS
ASPB Publications PLANT PHYSIOLOGY® THE PLANT CELL
Copyright © 1999 by the American Society of Plant Biologists