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Sucrose Synthase in Legume Nodules Is Essential for Nitrogen Fixation1

Anthony J. Gordon*, Frank R. Minchin, Caron L. James, and Olga Komina2

Department of Environmental Biology, Institute of Grassland and Environmental Research, Plas Gogerddan, Aberystwyth, Ceridigion SY23 3EB, United Kingdom

The role of sucrose synthase (SS) in the fixation of N was examined in the rug4 mutant of pea (Pisum sativum L.) plants in which SS activity was severely reduced. When dependent on nodules for their N supply, the mutant plants were not viable and appeared to be incapable of effective N fixation, although nodule formation was essentially normal. In fact, N and C resources invested in nodules were much greater in mutant plants than in the wild-type (WT) plants. Low SS activity in nodules (present at only 10% of WT levels) resulted in lower amounts of total soluble protein and leghemoglobin and lower activities of several enzymes compared with WT nodules. Alkaline invertase activity was not increased to compensate for reduced SS activity. Leghemoglobin was present at less than 20% of WT values, so O2 flux may have been compromised. The two components of nitrogenase were present at normal levels in mutant nodules. However, only a trace of nitrogenase activity was detected in intact plants and none was found in isolated bacteroids. The results are discussed in relation to the role of SS in the provision of C substrates for N fixation and in the development of functional nodules.

1   The Institute of Grassland and Environmental Research is funded by the Biotechnology and Biological Sciences Research Council of the United Kingdom. O.K. was supported by the Royal Society.
2   Present address: A.N. Bach Institute of Biochemistry, Leninsky Pr 33, Moscow 117071, Russia.
*   Corresponding author; e-mail tony.gordon{at}bbsrc.ac.uk; fax 44-9-70-828-357.

Plant Physiol. (1999) 120: 867-878
Copyright Clearance Center:   0032-0889/99/120//12
© 1999 American Society of Plant Physiologists




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