Biosynthesis of Ascorbic Acid in Kidney Bean. L-Galactono--Lactone Dehydrogenase Is an Intrinsic Protein Located at the Mitochondrial Inner Membrane¹

Emilio Siendones, José A. González-Reyes, Carlos Santos-Ocaña, Plácido Navas, and Francisco Córdoba^*

Departamento de Biología Celular, Facultad de Ciencias, Universidad de Córdoba, 14004 Córdoba, Spain (E.S., J.A.G.-R.); Departamento de Economía y Empresa, Facultad de Ciencias, Universidad Pablo de Olavide, 41013 Sevilla, Spain (C.S.-O., P.N.); and Departamento de Ciencias Agroforestales, Facultad de Ciencias Experimentales, Universidad de Huelva, 21819 Huelva, Spain (F.C.)

Hypocotyls of kidney beans (Phaseolus vulgaris L.) accumulated ascorbate after preincubation with a number of possible precursors, mainly L-galactono--lactone (L-GL) and L-gulono--lactone. The increase in the intracellular ascorbate concentration was parallel to the high stimulation of the L-GL dehydrogenase (L-GLD) activity measured in vitro using L-GL as a substrate and cytochrome c as an electron acceptor. Cell fractionation using a continuous linear Percoll gradient demonstrated that L-GLD is associated with mitochondria; therefore, pure mitochondria were isolated and subjected to detergent treatment to separate soluble from membrane-linked proteins. L-GLD activity was mainly associated with the detergent phase, suggesting that a membrane-intrinsic protein is responsible for the ascorbic acid biosynthetic activity. Subfractionation of mitochondria demonstrated that L-GLD is located at the inner membrane.

Plant Physiol. (1999) 120: 907-912
Copyright Clearance Center:   0032-0889/99/120//06
© 1999 American Society of Plant Physiologists

This article has been cited by other articles:

				Z. Chen and D. R. Gallie Dehydroascorbate Reductase Affects Non-photochemical Quenching and Photosynthetic Performance J. Biol. Chem., August 1, 2008; 283(31): 21347 - 21361. [Abstract] [Full Text] [PDF]

				A. Nunes-Nesi, R. Sulpice, Y. Gibon, and A. R. Fernie The enigmatic contribution of mitochondrial function in photosynthesis J. Exp. Bot., May 1, 2008; 59(7): 1675 - 1684. [Abstract] [Full Text] [PDF]

				J. Loscos, M. A. Matamoros, and M. Becana Ascorbate and Homoglutathione Metabolism in Common Bean Nodules under Stress Conditions and during Natural Senescence Plant Physiology, March 1, 2008; 146(3): 1282 - 1292. [Abstract] [Full Text] [PDF]

				M. A. Matamoros, J. Loscos, M. J. Coronado, J. Ramos, S. Sato, P. S. Testillano, S. Tabata, and M. Becana Biosynthesis of Ascorbic Acid in Legume Root Nodules Plant Physiology, July 1, 2006; 141(3): 1068 - 1077. [Abstract] [Full Text] [PDF]

				D. M. Rhoads, A. L. Umbach, C. C. Subbaiah, and J. N. Siedow Mitochondrial Reactive Oxygen Species. Contribution to Oxidative Stress and Interorganellar Signaling Plant Physiology, June 1, 2006; 141(2): 357 - 366. [Full Text] [PDF]

				C. G Bartoli, J. Yu, F. Gomez, L. Fernandez, L. McIntosh, and C. H Foyer Inter-relationships between light and respiration in the control of ascorbic acid synthesis and accumulation in Arabidopsis thaliana leaves J. Exp. Bot., May 1, 2006; 57(8): 1621 - 1631. [Abstract] [Full Text] [PDF]

				M. d. C. Cordoba-Pedregosa, J. M. Villalba, F. Cordoba, and J. A. Gonzalez-Reyes Changes in intracellular and apoplastic peroxidase activity, ascorbate redox status, and root elongation induced by enhanced ascorbate content in Allium cepa L. J. Exp. Bot., February 1, 2005; 56(412): 685 - 694. [Abstract] [Full Text] [PDF]

				T. Salusjarvi, N. Kalkkinen, and A. N. Miasnikov Cloning and Characterization of Gluconolactone Oxidase of Penicillium cyaneo-fulvum ATCC 10431 and Evaluation of Its Use for Production of D-Erythorbic Acid in Recombinant Pichia pastoris Appl. Envir. Microbiol., September 1, 2004; 70(9): 5503 - 5510. [Abstract] [Full Text] [PDF]

				I. Pateraki, M. Sanmartin, M. S. Kalamaki, D. Gerasopoulos, and A. K. Kanellis Molecular characterization and expression studies during melon fruit development and ripening of L-galactono-1,4-lactone dehydrogenase J. Exp. Bot., August 1, 2004; 55(403): 1623 - 1633. [Abstract] [Full Text] [PDF]

				A. H. Millar, V. Mittova, G. Kiddle, J. L. Heazlewood, C. G. Bartoli, F. L. Theodoulou, and C. H. Foyer Control of Ascorbate Synthesis by Respiration and Its Implications for Stress Responses Plant Physiology, October 1, 2003; 133(2): 443 - 447. [Full Text] [PDF]

				V. R. Franceschi and N. M. Tarlyn L-Ascorbic Acid Is Accumulated in Source Leaf Phloem and Transported to Sink Tissues in Plants Plant Physiology, October 1, 2002; 130(2): 649 - 656. [Abstract] [Full Text] [PDF]

				C. G. Bartoli, G. M. Pastori, and C. H. Foyer Ascorbate Biosynthesis in Mitochondria Is Linked to the Electron Transport Chain between Complexes III and IV Plant Physiology, May 1, 2000; 123(1): 335 - 344. [Abstract] [Full Text]

				M. W. Davey, C. Gilot, G. Persiau, J. Østergaard, Y. Han, G. C. Bauw, and M. C. Van Montagu Ascorbate Biosynthesis in Arabidopsis Cell Suspension Culture Plant Physiology, October 1, 1999; 121(2): 535 - 544. [Abstract] [Full Text]