The Endogenous Sulfated Pentapeptide Phytosulfokine- Stimulates Tracheary Element Differentiation of Isolated Mesophyll Cells of Zinnia¹

Yoshikatsu Matsubayashi^*, Leiko Takagi, Naomi Omura, Akiko Morita, and Youji Sakagami

Laboratory of Bioactive Natural Products Chemistry, Graduate School of Bioagricultural Sciences, Nagoya University, Chikusa, Nagoya 464-8601, Japan

Dispersed zinnia (Zinnia elegans) mesophyll cells cannot differentiate into tracheary elements (TEs) at low cell density conditions even if auxin and cytokinin are present in the medium, indicating the involvement of intercellular interactions during the initiation and/or subsequent progresses in TE differentiation. When zinnia cells were incubated at a low density (2.5 × 10⁴ cells mL¹) in TE-inductive medium in the presence of various concentrations of phytosulfokine (PSK)-, which was originally identified as an intercellular signal peptide involved in cell proliferation, TE differentiation was strongly stimulated in a dose-dependent fashion; more than 35% of the living cells differentiated into TEs by 5 d of culture in the presence of 10 nM PSK-. Enzyme-linked immunosorbent assay and mass spectroscopy confirmed that cultured zinnia cells produce nanomolar levels of PSKs under inductive conditions. These results suggest that PSK- is a factor responsible for TE differentiation of zinnia mesophyll cells.

Plant Physiol. (1999) 120: 1043-1048
Copyright Clearance Center:   0032-0889/99/120//06
© 1999 American Society of Plant Physiologists

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