Plant Physiol.
HOME HELP FEEDBACK SUBSCRIPTIONS ARCHIVE SEARCH TABLE OF CONTENTS
QUICK SEARCH:   [advanced]


     

This Article
Right arrow Full Text
Right arrow Full Text (PDF)
Right arrow Alert me when this article is cited
Right arrow Alert me if a correction is posted
Services
Right arrow Email this article to a friend
Right arrow Similar articles in this journal
Right arrow Similar articles in Web of Science
Right arrow Similar articles in PubMed
Right arrow Alert me to new issues of the journal
Right arrow Download to citation manager
Right arrow reprints & permissions
Citing Articles
Right arrow Citing Articles via HighWire
Right arrow Citing Articles via CrossRef
Right arrow Citing Articles via Web of Science (21)
Right arrow Citing Articles via Google Scholar
Google Scholar
Right arrow Articles by Goossens, A.
Right arrow Articles by Angenon, G.
Right arrow Search for Related Content
PubMed
Right arrow PubMed Citation
Right arrow Articles by Goossens, A.
Right arrow Articles by Angenon, G.
Agricola
Right arrow Articles by Goossens, A.
Right arrow Articles by Angenon, G.

The arcelin-5 Gene of Phaseolus vulgaris Directs High Seed-Specific Expression in Transgenic Phaseolus acutifolius and Arabidopsis Plants1

Alain Goossens2, Willy Dillen3, Janniek De Clercq, Marc Van Montagu*, and Geert Angenon

Laboratorium voor Genetica, Departement Plantengenetica, Vlaams Interuniversitair Instituut voor Biotechnologie, Universiteit Gent, B-9000 Gent, Belgium

The regulatory sequences of many genes encoding seed storage proteins have been used to drive seed-specific expression of a variety of proteins in transgenic plants. Because the levels at which these transgene-derived proteins accumulate are generally quite low, we investigated the utility of the arcelin-5 regulatory sequences in obtaining high seed-specific expression in transgenic plants. Arcelin-5 is an abundant seed protein found in some wild common bean (Phaseolus vulgaris L.) genotypes. Seeds of Arabidopsis and Tepary bean (Phaseolus acutifolius A. Gray) plants transformed with arcelin-5 gene constructs synthesized arcelin-5 to levels of 15% and 25% of the total protein content, respectively. To our knowledge, such high expression levels directed by a transgene have not been reported before. The transgenic plants also showed low plant-to-plant variation in arcelin expression. Complex transgene integration patterns, which often result in gene silencing effects, were not associated with reduced arcelin-5 expression. High transgene expression was the result of high mRNA steady-state levels and was restricted to seeds. This indicates that all requirements for high seed-specific expression are cis elements present in the cloned genomic arcelin-5 sequence and trans-acting factors that are available in Arabidopsis and Phaseolus spp., and thus probably in most dicotyledonous plants.

1   This work was supported in part by a grant from the Algemeen Bestuur voor Ontwikkelingssamenwerking. A.G. and J.D.C. both received fellowships from the Vlaams Instituut voor de bevordering van het Wetenschappelijk-Technologisch Onderzoek in de Industrie.
2   Present address: Instituto de Biología Molecular y Celular de Plantas, Universidad Politécnica de Valencia-Consejo Superior de Investigaciones Científicas, Camino de Vera s/n, E-46022 Valencia, Spain.
3   Present address: CropDesign N.V., Technologiepark 3, B-9052 Zwijnaarde, Belgium.
*   Corresponding author; e-mail mamon{at}gengenp.rug.ac.be; fax 32-9-2645349.

Plant Physiol. (1999) 120: 1095-1104
Copyright Clearance Center:   0032-0889/99/120//10
© 1999 American Society of Plant Physiologists




This article has been cited by other articles:


Home page
 Proc. Natl. Acad. Sci. USAHome page
B. Van Droogenbroeck, J. Cao, J. Stadlmann, F. Altmann, S. Colanesi, S. Hillmer, D. G. Robinson, E. Van Lerberge, N. Terryn, M. Van Montagu, et al.
Aberrant localization and underglycosylation of highly accumulating single-chain Fv-Fc antibodies in transgenic Arabidopsis seeds
PNAS, January 23, 2007; 104(4): 1430 - 1435.
[Abstract] [Full Text] [PDF]

Home page
 J Exp BotHome page
A. Goossens, C. Quintero, W. Dillen, R. De Rycke, J. Flower Valor, J. De Clercq, M. Van Montagu, C. Cardona, and G. Angenon
Analysis of bruchid resistance in the wild common bean accession G02771: no evidence for insecticidal activity of arcelin 5
J. Exp. Bot., July 1, 2000; 51(348): 1229 - 1236.
[Abstract] [Full Text] [PDF]


HOME HELP FEEDBACK SUBSCRIPTIONS ARCHIVE SEARCH TABLE OF CONTENTS
ASPB Publications PLANT PHYSIOLOGY® THE PLANT CELL
Copyright © 1999 by the American Society of Plant Biologists