Plant Physiol. Illumina
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Characterization of Two Photosynthetic Mutants of Maize1

Donald A. Heck, Donald Miles, and Parag R. Chitnis*

Department of Biochemistry, Biophysics and Molecular Biology, Iowa State University, Ames, Iowa, 50011 (D.A.H., P.R.C.); and Division of Biological Sciences, University of Columbia, Columbia, Missouri 65211 (D.M.)

We describe here the biochemical characteristics of the hcf44 and hcf47 (high chlorophyll fluorescence) mutants of maize (Zea mays L.). Both mutants were sensitive to high light intensities, exhibiting reduced growth and fluorescence intensity. Electron transport through the mutants' photosystem (PS) I and PSII reaction centers was reduced and NADP+ photoreduction was absent. Western analysis revealed that the hcf44 mutant was missing some or all of the PsaC, PsaD, and PsaE polypeptides of the PSI reaction center, and reverse transcriptase-polymerase chain reaction demonstrated that this loss was the result of a posttranscriptional event. The hcf47 mutant had reduced levels of many PSI and PSII polypeptides. These data indicate a possible defect in the synthesis or assembly of the PsaC subunit in the hcf44 mutant, whereas the hcf47 mutant may have a more general defect in the biogenesis of photosynthetic membranes. Our results demonstrate the coordinated assembly of the peripheral proteins into the PSI complexes of higher plants and demonstrate the in vivo requirement of PsaC, PsaD, and PsaE subunits for the function of PSI in higher plants.

1   This work was supported partially by the U.S. Department of Agriculture-National Research Initiative Competitive Grants Program (grant no. 97-35306-4555) and the National Science Foundation (grant no. MCB9723001). This is journal paper no. J-18286 of the Iowa Agriculture and Home Economics Experiment Station, Ames, project nos. 3,416 and 3,496, and was supported by the Hatch Act and by State of Iowa funds.
*   Corresponding author; e-mail chitnis{at}iastate.edu; fax 515-294-0453.

Plant Physiol. (1999) 120: 1129-1136
Copyright Clearance Center:   0032-0889/99/120//08
© 1999 American Society of Plant Physiologists




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