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Characterization of MdMADS2, a Member of the
SQUAMOSA Subfamily of Genes, in Apple1
Soon-Kee Sung,
Gyung-Hee Yu, and
Gynheung An*
Department of Life Science, Pohang University of Science and
Technology, Pohang 790-784, Republic of Korea
A MADS-box gene,
MdMADS2, was isolated from the apple
(Malus × domestica Borkh.) var Fuji
and its developmental expression pattern was studied during flower
development. MdMADS2 shares a high degree of amino acid
sequence identity with the SQUAMOSA subfamily of genes.
RNA blot analysis showed that MdMADS2 is transcribed through all stages of flower development, and its transcription was
seen in the four floral organs. RNA in situ hybridization revealed that
the MdMADS2 mRNA is expressed both in the inflorescence meristem and in the floral meristem. The MdMADS2
transcript was detected at all stages of flower development. Protein
localization analysis showed that MdMADS2 protein was excluded from the
stamen and carpel primordia, in which a considerable
MdMADS2 mRNA signal was detected. This indicates that
posttanscriptional regulation may be involved in the
MdMADS2-mediated control of flower development. Transgenic tobacco expressing the MdMADS2 gene from the
cauliflower mosaic virus 35S promoter showed early flowering and
shorter bolts, but did not show any homeotic changes in the floral
organs. These results suggest that MdMADS2 plays an
important role during early stages of flower development.
1
This work was supported in part by a special
grant research program of the Ministry of Agriculture and Forestry of
Korea to G.A. S.-K.S. was the recipient of postdoctoral research
fellowships from the Korea Science and Engineering Foundation and the
Korea Research Foundation.
*
Corresponding author; e-mail genean{at}postech.ac.kr; fax
82-56-22-79-21-99.
Plant Physiol. (1999) 120: 969-978
Copyright Clearance Center: 0032-0889/99/120//10
© 1999 American Society of Plant Physiologists
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