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Biochemical Characterization of the Suberization-Associated
Anionic Peroxidase of Potato1
Mark A. Bernards2, *,
Warren D. Fleming3,
David B. Llewellyn4,
Ronny Priefer4,
Xiaolong Yang2,
Anita Sabatino, and
Guy L. Plourde
Program in Chemistry, University of Northern British Columbia, 3333 University Way, Prince George, British Columbia, Canada V2N 4Z9
The
anionic peroxidase associated with the suberization response in potato
(Solanum tuberosum L.) tubers during wound healing has
been purified and partially characterized at the biochemical level. It
is a 45-kD, class III (plant secretory) peroxidase that is localized to
suberizing tissues and shows a preference for feruloyl
(o-methoxyphenol)-substituted substrates (order of
substrate preference: feruloyl > caffeoyl > p-coumaryl  syringyl) such as those that
accumulate in tubers during wound healing. There was little influence
on oxidation by side chain derivatization, although hydroxycinnamates
were preferred over the corresponding hydroxycinnamyl alcohols. The
substrate specificity pattern is consistent with the natural substrate
incorporation into potato wound suberin. In contrast, the cationic
peroxidase(s) induced in response to wound healing in potato tubers is
present in both suberizing and nonsuberizing tissues and does not
discriminate between hydroxycinnamates and hydroxycinnamyl alcohols. A
synthetic polymer prepared using
E-[8-13C]ferulic acid,
H2O2, and the purified anionic enzyme contained a significant amount of cross-linking through C-8, albeit with retention of unsaturation.
1
This research was supported by a Natural
Sciences and Engineering Research Council of Canada (NSERC) operating
grant to M.A.B. D.B.L. was supported in part by a NSERC
undergraduate scholarship.
2
Present address: Department of Plant Sciences,
University of Western Ontario, London, ON, Canada N6A 5B7.
3
Present address: Department of Biology,
University of Alberta, Edmonton, AB, Canada T6G 2E9.
4
Present address: Department of Chemistry, McGill
University, Montreal, PQ, Canada H3A 1B1.
*
Corresponding author; e-mail bernards{at}julian.uwo.ca; fax
519-661-3935.
Plant Physiol. (1999) 121: 135-146
Copyright Clearance Center: 0032-0889/99/121//12
© 1999 American Society of Plant Physiologists
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