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Hydrogen Peroxide from the Oxidative Burst Is Neither Necessary Nor Sufficient for Hypersensitive Cell Death Induction, Phenylalanine Ammonia Lyase Stimulation, Salicylic Acid Accumulation, or Scopoletin Consumption in Cultured Tobacco Cells Treated with Elicitin

Stéphan Dorey1, Marguerite Kopp, Pierrette Geoffroy, Bernard Fritig, and Serge Kauffmann*

Institut de Biologie Moléculaire des Plantes du Centre National de la Recherche Scientifique, Université Louis Pasteur, 12 rue du Général Zimmer, 67084 Strasbourg cedex, France

H2O2 from the oxidative burst, cell death, and defense responses such as the production of phenylalanine ammonia lyase (PAL), salicylic acid (SA), and scopoletin were analyzed in cultured tobacco (Nicotiana tabacum) cells treated with three proteinaceous elicitors: two elicitins (alpha -megaspermin and beta -megaspermin) and one glycoprotein. These three proteins have been isolated from Phytophthora megasperma H20 and have been previously shown to be equally efficient in inducing a hypersensitive response (HR) upon infiltration into tobacco leaves. However, in cultured tobacco cells these elicitors exhibited strikingly different biological activities. beta -Megaspermin was the only elicitor that caused cell death and induced a strong, biphasic H2O2 burst. Both elicitins stimulated PAL activity similarly and strongly, while the glycoprotein caused only a slight increase. Only elicitins induced SA accumulation and scopoletin consumption, and beta -megaspermin was more efficient. To assess the role of H2O2 in HR cell death and defense response expression in elicitin-treated cells, a gain and loss of function strategy was used. Our results indicated that H2O2 was neither necessary nor sufficient for HR cell death, PAL activation, or SA accumulation, and that extracellular H2O2 was not a direct cause of intracellular scopoletin consumption.

1   S.D. was supported by a doctoral fellowship of the French Ministry of Research.
*   Corresponding author; e-mail serge.kauffmann{at}ibmp-ulp.u-strasbg.fr; fax 33-388-61-4442.

Plant Physiol. (1999) 121: 163-172
Copyright Clearance Center:   0032-0889/99/121//10
© 1999 American Society of Plant Physiologists




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