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Gene-Specific Changes in alpha -Tubulin Transcript Accumulation in Developing Cotton Fibers1

David J. Whittaker and Barbara A. Triplett*

Cotton Fiber Bioscience, United States Department of Agriculture-Agricultural Research Service, Southern Regional Research Center, 1100 Robert E. Lee Boulevard, New Orleans, Louisiana 70124

The fibers of cotton (Gossypium hirsutum) are single-cell trichomes that undergo rapid and synchronous elongation. Cortical microtubules provide spatial information necessary for the alignment of cellulose microfibrils that confine and regulate cell elongation. We used gene-specific probes to investigate alpha -tubulin transcript levels in elongating cotton fibers. Two discrete patterns of transcript accumulation were observed. Whereas transcripts of alpha -tubulin genes GhTua2/3 and GhTua4 increased in abundance from 10 to 20 d post anthesis (DPA), GhTua1 and GhTua5 transcripts were abundant only through to 14 DPA, and dropped significantly at 16 DPA with the onset of secondary wall synthesis. This is the first report, to our knowledge, of gene-specific changes in tubulin transcript levels during the development of a terminally differentiated plant cell. The decrease in abundance of GhTua1 and GhTua5 transcripts was correlated with pronounced changes in cell wall structure, suggesting that alpha -tubulin isoforms may be functionally distinct in elongating fiber cells. Although total alpha -tubulin transcript levels were much higher in fiber than several other tissues, including the hypocotyl and pollen, none of the alpha -tubulins was specific to fiber cells.

1   This work was supported by the U.S. Department of Agriculture, Agricultural Research Service, Current Research Information System project no. 6435-21440-0001-00D.
*   Corresponding author; e-mail btriplet{at}nola.srrc.usda.gov; fax 504-286-4419.

Plant Physiol. (1999) 121: 181-188
Copyright Clearance Center:   0032-0889/99/121//08
© 1999 American Society of Plant Physiologists




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