Plant Physiol. email content delivery
HOME HELP FEEDBACK SUBSCRIPTIONS ARCHIVE SEARCH TABLE OF CONTENTS
QUICK SEARCH:   [advanced]


     

This Article
Right arrow Full Text
Right arrow Full Text (PDF)
Right arrow Alert me when this article is cited
Right arrow Alert me if a correction is posted
Services
Right arrow Email this article to a friend
Right arrow Similar articles in this journal
Right arrow Similar articles in Web of Science
Right arrow Similar articles in PubMed
Right arrow Alert me to new issues of the journal
Right arrow Download to citation manager
Right arrow reprints & permissions
Citing Articles
Right arrow Citing Articles via HighWire
Right arrow Citing Articles via CrossRef
Right arrow Citing Articles via Web of Science (67)
Right arrow Citing Articles via Google Scholar
Google Scholar
Right arrow Articles by Maury, S.
Right arrow Articles by Legrand, M.
Right arrow Search for Related Content
PubMed
Right arrow PubMed Citation
Right arrow Articles by Maury, S.
Right arrow Articles by Legrand, M.
Agricola
Right arrow Articles by Maury, S.
Right arrow Articles by Legrand, M.

Tobacco O-Methyltransferases Involved in Phenylpropanoid Metabolism. The Different Caffeoyl-Coenzyme A/5-Hydroxyferuloyl-Coenzyme A 3/5-O-Methyltransferase and Caffeic Acid/5-Hydroxyferulic Acid 3/5-O-Methyltransferase Classes Have Distinct Substrate Specificities and Expression Patterns1

Stéphane Maury, Pierrette Geoffroy, and Michel Legrand*

Institut de Biologie Moléculaire des Plantes, Centre National de la Recherche Scientifique, Université Louis Pasteur, 12 rue du Général Zimmer, 67084 Strasbourg cedex, France

The biosynthesis of lignin monomers involves two methylation steps catalyzed by orthodiphenol-O-methyltransferases: caffeic acid/5-hydroxyferulic acid 3/5-O-methyltransferases (COMTs) and caffeoyl-coenzyme A (CoA)/5-hydroxyferuloyl-CoA 3/5-O-methyltransferases (CCoAOMTs). Two COMT classes (I and II) were already known to occur in tobacco (Nicotiana tabacum) and three distinct CCoAOMT classes have now been characterized. These three CCoAOMT classes displayed a maximum level of expression at different stages of stem development, in accordance with their involvement in the synthesis of lignin guaiacyl units. Expression profiles upon tobacco mosaic virus infection of tobacco leaves revealed a biphasic pattern of induction for COMT I, COMT II, and CCoAOMTs. The different isoforms were expressed in Escherichia coli and our results showed that CCoAOMTs and, more surprisingly, COMTs efficiently methylated hydroxycinnamoyl-CoA esters. COMT I was also active toward 5-hydroxyconiferyl alcohol, indicating that COMT I that catalyzes syringyl unit synthesis in planta may operate at the free acid, CoA ester, or alcohol levels. COMT II that is highly inducible by infection also accepted caffeoyl-CoA as a substrate, thus suggesting a role in ferulate derivative deposition in the walls of infected cells. Tobacco appears to possess an array of O-methyltransferase isoforms with variable efficiency toward the diverse plant o-diphenolic substrates.

1   This work was supported by the Ministère de l'Education Nationale, de l'Enseignement Supérieur et de la Recherche (grant no. ACC-SV14) and by the Commission of European Communities (FAIR-TIMBER grant no. CT 95-0424).
*   Corresponding author; e-mail michel.legrand{at}ibmp-ulp.u-strasbg.fr; fax: 33-3-88-61-44-42.

Plant Physiol. (1999) 121: 215-224
Copyright Clearance Center:   0032-0889/99/121//10
© 1999 American Society of Plant Physiologists




This article has been cited by other articles:


Home page
 GeneticsHome page
Y. Han, K. Gasic, and S. S. Korban
Multiple-Copy Cluster-Type Organization and Evolution of Genes Encoding O-Methyltransferases in the Apple
Genetics, August 1, 2007; 176(4): 2625 - 2635.
[Abstract] [Full Text] [PDF]

Home page
 Mol. Cell. ProteomicsHome page
N. Imin, T. Kerim, J. J. Weinman, and B. G. Rolfe
Low Temperature Treatment at the Young Microspore Stage Induces Protein Changes in Rice Anthers
Mol. Cell. Proteomics, February 1, 2006; 5(2): 274 - 292.
[Abstract] [Full Text] [PDF]

Home page
 Plant Physiol.Home page
D. Vincent, C. Lapierre, B. Pollet, G. Cornic, L. Negroni, and M. Zivy
Water Deficits Affect Caffeate O-Methyltransferase, Lignification, and Related Enzymes in Maize Leaves. A Proteomic Investigation
Plant Physiology, March 1, 2005; 137(3): 949 - 960.
[Abstract] [Full Text] [PDF]

Home page
 Plant Physiol.Home page
K. Morreel, J. Ralph, F. Lu, G. Goeminne, R. Busson, P. Herdewijn, J. L. Goeman, J. Van der Eycken, W. Boerjan, and E. Messens
Phenolic Profiling of Caffeic Acid O-Methyltransferase-Deficient Poplar Reveals Novel Benzodioxane Oligolignols
Plant Physiology, December 1, 2004; 136(4): 4023 - 4036.
[Abstract] [Full Text] [PDF]

Home page
 Plant CellHome page
L. Hoffmann, S. Besseau, P. Geoffroy, C. Ritzenthaler, D. Meyer, C. Lapierre, B. Pollet, and M. Legrand
Silencing of Hydroxycinnamoyl-Coenzyme A Shikimate/Quinate Hydroxycinnamoyltransferase Affects Phenylpropanoid Biosynthesis
PLANT CELL, June 1, 2004; 16(6): 1446 - 1465.
[Abstract] [Full Text] [PDF]

Home page
 J. Biol. Chem.Home page
M. Ibdah, X.-H. Zhang, J. Schmidt, and T. Vogt
A Novel Mg2+-dependent O-Methyltransferase in the Phenylpropanoid Metabolism of Mesembryanthemum crystallinum
J. Biol. Chem., November 7, 2003; 278(45): 43961 - 43972.
[Abstract] [Full Text] [PDF]

Home page
 Plant Physiol.Home page
J. Raes, A. Rohde, J. H. Christensen, Y. Van de Peer, and W. Boerjan
Genome-Wide Characterization of the Lignification Toolbox in Arabidopsis
Plant Physiology, November 1, 2003; 133(3): 1051 - 1071.
[Abstract] [Full Text] [PDF]

Home page
 J. Biol. Chem.Home page
L. Hoffmann, S. Maury, F. Martz, P. Geoffroy, and M. Legrand
Purification, Cloning, and Properties of an Acyltransferase Controlling Shikimate and Quinate Ester Intermediates in Phenylpropanoid Metabolism
J. Biol. Chem., January 3, 2003; 278(1): 95 - 103.
[Abstract] [Full Text] [PDF]

Home page
 Plant Physiol.Home page
R. B. Nair, Q. Xia, C. J. Kartha, E. Kurylo, R. N. Hirji, R. Datla, and G. Selvaraj
Arabidopsis CYP98A3 Mediating Aromatic 3-Hydroxylation. Developmental Regulation of the Gene, and Expression in Yeast
Plant Physiology, September 1, 2002; 130(1): 210 - 220.
[Abstract] [Full Text] [PDF]

Home page
 Plant CellHome page
J. Chong, R. Baltz, C. Schmitt, R. Beffa, B. Fritig, and P. Saindrenan
Downregulation of a Pathogen-Responsive Tobacco UDP-Glc:Phenylpropanoid Glucosyltransferase Reduces Scopoletin Glucoside Accumulation, Enhances Oxidative Stress, and Weakens Virus Resistance
PLANT CELL, May 1, 2002; 14(5): 1093 - 1107.
[Abstract] [Full Text] [PDF]

Home page
 Plant Physiol.Home page
G. Pinçon, M. Chabannes, C. Lapierre, B. Pollet, K. Ruel, J.-P. Joseleau, A. M. Boudet, and M. Legrand
Simultaneous Down-Regulation of Caffeic/5-Hydroxy Ferulic Acid-O-Methyltransferase I and Cinnamoyl-Coenzyme A Reductase in the Progeny from a Cross between Tobacco Lines Homozygous for Each Transgene. Consequences for Plant Development and Lignin Synthesis
Plant Physiology, May 1, 2001; 126(1): 145 - 155.
[Abstract] [Full Text]

Home page
 Plant Physiol.Home page
O. Klarzynski, B. Plesse, J.-M. Joubert, J.-C. Yvin, M. Kopp, B. Kloareg, and B. Fritig
Linear beta -1,3 Glucans Are Elicitors of Defense Responses in Tobacco
Plant Physiology, November 1, 2000; 124(3): 1027 - 1038.
[Abstract] [Full Text]

Home page
 Plant Physiol.Home page
R. Zhong, W. H. Morrison III, D. S. Himmelsbach, F. L. Poole II, and Z.-H. Ye
Essential Role of Caffeoyl Coenzyme A O-Methyltransferase in Lignin Biosynthesis in Woody Poplar Plants
Plant Physiology, October 1, 2000; 124(2): 563 - 578.
[Abstract] [Full Text]

Home page
 J. Biol. Chem.Home page
H. Meyermans, K. Morreel, C. Lapierre, B. Pollet, A. De Bruyn, R. Busson, P. Herdewijn, B. Devreese, J. Van Beeumen, J. M. Marita, et al.
Modifications in Lignin and Accumulation of Phenolic Glucosides in Poplar Xylem upon Down-regulation of Caffeoyl-Coenzyme A O-Methyltransferase, an Enzyme Involved in Lignin Biosynthesis
J. Biol. Chem., November 17, 2000; 275(47): 36899 - 36909.
[Abstract] [Full Text] [PDF]

Home page
 J. Biol. Chem.Home page
G. Schoch, S. Goepfert, M. Morant, A. Hehn, D. Meyer, P. Ullmann, and D. Werck-Reichhart
CYP98A3 from Arabidopsis thaliana Is a 3'-Hydroxylase of Phenolic Esters, a Missing Link in the Phenylpropanoid Pathway
J. Biol. Chem., September 21, 2001; 276(39): 36566 - 36574.
[Abstract] [Full Text] [PDF]

Home page
 J. Biol. Chem.Home page
L. Hoffmann, S. Maury, M. Bergdoll, L. Thion, M. Erard, and M. Legrand
Identification of the Enzymatic Active Site of Tobacco Caffeoyl-coenzyme A O-Methyltransferase by Site-directed Mutagenesis
J. Biol. Chem., September 21, 2001; 276(39): 36831 - 36838.
[Abstract] [Full Text] [PDF]

Home page
 Plant CellHome page
D. R. Gang, N. Lavid, C. Zubieta, F. Chen, T. Beuerle, E. Lewinsohn, J. P. Noel, and E. Pichersky
Characterization of Phenylpropene O-Methyltransferases from Sweet Basil: Facile Change of Substrate Specificity and Convergent Evolution within a Plant O-Methyltransferase Family
PLANT CELL, February 1, 2002; 14(2): 505 - 519.
[Abstract] [Full Text] [PDF]


HOME HELP FEEDBACK SUBSCRIPTIONS ARCHIVE SEARCH TABLE OF CONTENTS
ASPB Publications PLANT PHYSIOLOGY® THE PLANT CELL
Copyright © 1999 by the American Society of Plant Biologists