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Mechanically Induced Avoidance Response of Chloroplasts in Fern
Protonemal Cells1
Yoshikatsu Sato,
Akeo Kadota*, and
Masamitsu Wada
Department of Biological Sciences, Graduate School of Science,
Tokyo Metropolitan University, Minami-Osawa 1-1, Hachioji, Tokyo
192-0397, Japan
Cell response to mechanical
stimulation was investigated at a subcellular level in protonemal cells
of the fern Adiantum capillus-veneris L. by pressing a
small part of the cell with a microcapillary. In cells receiving local
stimulation, the chloroplasts moved away from the site of stimulation,
whereas the nuclei failed to show such avoidance movement. Mechanical
stimulation for a period as short as 0.3 min was enough to induce the
avoidance response to a maximal level. The avoidance movement of
chloroplasts started within 30 min and the plateau level of avoidance
was attained around 2 h after stimulation. By tracing the movement
of chloroplasts during the response, it was shown that the mobility of
chloroplasts near the stimulation site increased transiently within
1 h after the stimulation. After 2 to 3 h, it slowed down to
the control level without stimulation. The avoidance response was
inhibited by 0.1 mM cytochalasin B and 25 mM
2,3-butanedione monoxime but not by 3.3 µM
amiprophosmethyl or 5 mM colchicine. These findings indicate that the protonemal cells were very sensitive to mechanical stimulation and that chloroplasts moved away from the mechanically stimulated site through the actomyosin motile system.
1
This work was supported by Grants-in-Aid for
Scientific Research (to A.K. and M.W.) from the Ministry of Education,
Science, Sports and Culture (Japan).
*
Corresponding author; e-mail kadota-akeo{at}c.metro-u.ac.jp; fax
81-426-77-2559.
Plant Physiol. (1999) 121: 37-44
Copyright Clearance Center: 0032-0889/99/121//08
© 1999 American Society of Plant Physiologists
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