|
Root Formation in Ethylene-Insensitive Plants1
David G. Clark*,
Erika K. Gubrium,
James E. Barrett,
Terril A. Nell, and
Harry J. Klee
Environmental Horticulture Department, P.O. Box 110670 (D.G.C.,
E.K.G., J.E.B., T.A.N.), and Horticultural Sciences Department, P.O.
Box 110690 (H.J.K.), University of Florida, Gainesville, Florida
32611-0670
Experiments
with ethylene-insensitive tomato (Lycopersicon
esculentum) and petunia (Petunia × hybrida) plants were conducted to determine if normal or
adventitious root formation is affected by ethylene insensitivity.
Ethylene-insensitive Never ripe (NR) tomato plants
produced more belowground root mass but fewer aboveground adventitious roots than wild-type Pearson plants. Applied auxin (indole-3-butyric acid) increased adventitious root formation on
vegetative stem cuttings of wild-type plants but had little or no
effect on rooting of NR plants. Reduced adventitious root formation was
also observed in ethylene-insensitive transgenic petunia plants.
Applied 1-aminocyclopropane-1-carboxylic acid increased adventitious
root formation on vegetative stem cuttings from NR and wild-type
plants, but NR cuttings produced fewer adventitious roots than
wild-type cuttings. These data suggest that the promotive effect of
auxin on adventitious rooting is influenced by ethylene responsiveness.
Seedling root growth of tomato in response to mechanical impedance was
also influenced by ethylene sensitivity. Ninety-six percent of
wild-type seedlings germinated and grown on sand for 7 d grew
normal roots into the medium, whereas 47% of NR seedlings displayed
elongated taproots, shortened hypocotyls, and did not penetrate the
medium. These data indicate that ethylene has a critical role in
various responses of roots to environmental stimuli.
1
This work was supported in part by the Fred C. Gloeckner Foundation. This paper is University of Florida Journal
Series no. R-06707.
*
Corresponding author; e-mail dgc{at}gnv.ifas.ufl.edu; fax
352-392-3870.
Plant Physiol. (1999) 121: 53-60
Copyright Clearance Center: 0032-0889/99/121//08
© 1999 American Society of Plant Physiologists
This article has been cited by other articles:
|
|
|
|
 
M. L. Tucker, P. Xue, and R. Yang
1-Aminocyclopropane-1-carboxylic acid (ACC) concentration and ACC synthase expression in soybean roots, root tips, and soybean cyst nematode (Heterodera glycines)-infected roots
J. Exp. Bot.,
October 27, 2009;
(2009)
erp317v1.
[Abstract]
[Full Text]
[PDF]
|
|
|
|
|
|
|
Z. Lin, L. Arciga-Reyes, S. Zhong, L. Alexander, R. Hackett, I. Wilson, and D. Grierson
SlTPR1, a tomato tetratricopeptide repeat protein, interacts with the ethylene receptors NR and LeETR1, modulating ethylene and auxin responses and development
J. Exp. Bot.,
November 1, 2008;
59(15):
4271 - 4287.
[Abstract]
[Full Text]
[PDF]
|
|
|
|
|
|
|
A. Leblanc, H. Renault, J. Lecourt, P. Etienne, C. Deleu, and E. Le Deunff
Elongation Changes of Exploratory and Root Hair Systems Induced by Aminocyclopropane Carboxylic Acid and Aminoethoxyvinylglycine Affect Nitrate Uptake and BnNrt2.1 and BnNrt1.1 Transporter Gene Expression in Oilseed Rape
Plant Physiology,
April 1, 2008;
146(4):
1928 - 1940.
[Abstract]
[Full Text]
[PDF]
|
|
|
|
|
|
|
A. Hahn, R. Zimmermann, D. Wanke, K. Harter, and H. G. Edelmann
The Root Cap Determines Ethylene-Dependent Growth and Development in Maize Roots
Mol Plant,
March 1, 2008;
1(2):
359 - 367.
[Abstract]
[Full Text]
[PDF]
|
|
|
|
|
|
|
D. P. Puthoff, M. L. Ehrenfried, B. T. Vinyard, and M. L. Tucker
GeneChip profiling of transcriptional responses to soybean cyst nematode, Heterodera glycines, colonization of soybean roots
J. Exp. Bot.,
September 1, 2007;
58(12):
3407 - 3418.
[Abstract]
[Full Text]
[PDF]
|
|
|
|
|
|
|
R. Swarup, P. Perry, D. Hagenbeek, D. Van Der Straeten, G. T.S. Beemster, G. Sandberg, R. Bhalerao, K. Ljung, and M. J. Bennett
Ethylene Upregulates Auxin Biosynthesis in Arabidopsis Seedlings to Enhance Inhibition of Root Cell Elongation
PLANT CELL,
July 1, 2007;
19(7):
2186 - 2196.
[Abstract]
[Full Text]
[PDF]
|
|
|
|
|
|
|
M Kock, I Stenzel, and A Zimmer
Tissue-specific expression of tomato Ribonuclease LX during phosphate starvation-induced root growth
J. Exp. Bot.,
November 1, 2006;
57(14):
3717 - 3726.
[Abstract]
[Full Text]
[PDF]
|
|
|
|
|
|
|
C. S. Barry and J. J. Giovannoni
From The Cover: Ripening in the tomato Green-ripe mutant is inhibited by ectopic expression of a protein that disrupts ethylene signaling
PNAS,
May 16, 2006;
103(20):
7923 - 7928.
[Abstract]
[Full Text]
[PDF]
|
|
|
|
|
|
|
T. Kuroha, C. Ueguchi, H. Sakakibara, and S. Satoh
Cytokinin Receptors are Required for Normal Development of Auxin-transporting Vascular Tissues in the Hypocotyl but not in Adventitious Roots
Plant Cell Physiol.,
February 1, 2006;
47(2):
234 - 243.
[Abstract]
[Full Text]
[PDF]
|
|
|
|
|
|
|
Y. Liang and J. M. Harris
Response of root branching to abscisic acid is correlated with nodule formation both in legumes and nonlegumes
Am. J. Botany,
October 1, 2005;
92(10):
1675 - 1683.
[Abstract]
[Full Text]
[PDF]
|
|
|
|
|
|
|
B. A. Underwood, D. M. Tieman, K. Shibuya, R. J. Dexter, H. M. Loucas, A. J. Simkin, C. A. Sims, E. A. Schmelz, H. J. Klee, and D. G. Clark
Ethylene-Regulated Floral Volatile Synthesis in Petunia Corollas
Plant Physiology,
May 1, 2005;
138(1):
255 - 266.
[Abstract]
[Full Text]
[PDF]
|
|
|
|
|
|
|
N. N. Wang, M.-C. Shih, and N. Li
The GUS reporter-aided analysis of the promoter activities of Arabidopsis ACC synthase genes AtACS4, AtACS5, and AtACS7 induced by hormones and stresses
J. Exp. Bot.,
March 1, 2005;
56(413):
909 - 920.
[Abstract]
[Full Text]
[PDF]
|
|
|
|
|
|
|
K. Shibuya, K. G. Barry, J. A. Ciardi, H. M. Loucas, B. A. Underwood, S. Nourizadeh, J. R. Ecker, H. J. Klee, and D. G. Clark
The Central Role of PhEIN2 in Ethylene Responses throughout Plant Development in Petunia
Plant Physiology,
October 1, 2004;
136(2):
2900 - 2912.
[Abstract]
[Full Text]
[PDF]
|
|
|
|
|
|
|
H. J. Klee
Ethylene Signal Transduction. Moving beyond Arabidopsis
Plant Physiology,
June 1, 2004;
135(2):
660 - 667.
[Full Text]
[PDF]
|
|
|
|
|
|
|
D. Tholen, L. A.C.J. Voesenek, and H. Poorter
Ethylene Insensitivity Does Not Increase Leaf Area or Relative Growth Rate in Arabidopsis, Nicotiana tabacum, and Petunia x hybrida
Plant Physiology,
April 1, 2004;
134(4):
1803 - 1812.
[Abstract]
[Full Text]
[PDF]
|
|
|
|
|
|
|
H. ZAID, R. EL MORABET, H. G. DIEM, and M. ARAHOU
Does Ethylene Mediate Cluster Root Formation under Iron Deficiency?
Ann. Bot.,
November 1, 2003;
92(5):
673 - 677.
[Abstract]
[Full Text]
[PDF]
|
|
|
|
|
|
|
J. Harris
Shedding light on an underground problem
PNAS,
November 12, 2002;
99(23):
14616 - 14618.
[Full Text]
[PDF]
|
|
|
|
|
|
|
T. Kuroha, H. Kato, T. Asami, S. Yoshida, H. Kamada, and S. Satoh
A trans-zeatin riboside in root xylem sap negatively regulates adventitious root formation on cucumber hypocotyls
J. Exp. Bot.,
November 1, 2002;
53(378):
2193 - 2200.
[Abstract]
[Full Text]
[PDF]
|
|
|
|
|
|
|
H. J. Klee
Control of ethylene-mediated processes in tomato at the level of receptors
J. Exp. Bot.,
October 1, 2002;
53(377):
2057 - 2063.
[Abstract]
[Full Text]
[PDF]
|
|
|
|
|
|
|
J. Diaz, A. ten Have, and J. A.L. van Kan
The Role of Ethylene and Wound Signaling in Resistance of Tomato to Botrytis cinerea
Plant Physiology,
July 1, 2002;
129(3):
1341 - 1351.
[Abstract]
[Full Text]
[PDF]
|
|
|
|
|
|
|
B. K. Zolman, A. Yoder, and B. Bartel
Genetic Analysis of Indole-3-butyric Acid Responses in Arabidopsis thaliana Reveals Four Mutant Classes
Genetics,
November 1, 2000;
156(3):
1323 - 1337.
[Abstract]
[Full Text]
|
|
|
|
|
|
|
I. Llop-Tous, C. S. Barry, and D. Grierson
Regulation of Ethylene Biosynthesis in Response to Pollination in Tomato Flowers
Plant Physiology,
July 1, 2000;
123(3):
971 - 978.
[Abstract]
[Full Text]
|
|
|
|
|
