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Arabidopsis Mutants Lacking the 43- and 54-Kilodalton
Subunits of the Chloroplast Signal Recognition Particle
Have Distinct Phenotypes1
Pinky Amin,
Donna A.C. Sy,
Marsha L. Pilgrim2,
Devin H. Parry3,
Laurent Nussaume, and
Neil E. Hoffman4, *
Department of Plant Biology, Carnegie Institution of Washington,
260 Panama Street, Stanford, California 94305 (P.A., D.A.C.S., M.L.P.,
D.H.P., N.E.H.); and Departement d'Ecophysiologie Vegetale et de
Microbiologie Commissariat à l'Energie Atomique/Cadarache,
F-13108 St. Paul lez Durance cedex, France (L.N.)
The
chloroplast signal recognition particle (cpSRP) is a protein complex
consisting of 54- and 43-kD subunits encoded by the fifty-four
chloroplast, which encodes cpSRP54 (ffc),
and chaos (cao) loci, respectively. Two
new null alleles in the ffc locus have been identified.
ffc1-1 is caused by a stop codon in exon 10, while
ffc1-2 has a large DNA insertion in intron 8. ffc mutants have yellow first true leaves that
subsequently become green. The reaction center proteins D1, D2, and
psaA/B, as well as seven different light-harvesting chlorophyll
proteins (LHCPs), were found at reduced levels in the young
ffc leaves but at wild-type levels in the older leaves.
The abundance of the two types of LHCP was unaffected by the mutation,
while two others were increased in the absence of cpSRP54. Null mutants
in the cao locus contain reduced levels of the same
subset of LHCP proteins as ffc mutants, but are
distinguishable in four ways: young leaves are greener, the chlorophyll
a/b ratio is elevated, levels of reaction center proteins are normal, and there is no recovery in the level of LHCPs in
the adult plant. The data suggest that cpSRP54 and cpSRP43 have some
nonoverlapping roles and that alternative transport pathways can
compensate for the absence of a functional cpSRP.
1
This work was supported by grant no. GM42609-02
from the U.S. Department of Agriculture to N.E.H. This is Carnegie
Institution of Washington publication no. 1,411.
2
Present address: Mendel Biotechnology, 21375 Cabot Boulevard, Hayward, CA 94545.
3
Present address: Department of Biochemistry and
Biophysics, University of California, San Francisco, CA
94143-0448.
4
Present address: Paradigm Genetics, 104 Alexander Drive, P.O. Box 14528, Research Triangle Park, NC 27709.
*
Corresponding author; e-mail nhoffman{at}paragen.com; fax
919-381-1234.
Plant Physiol. (1999) 121: 61-70
Copyright Clearance Center: 0032-0889/99/121//10
© 1999 American Society of Plant Physiologists
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