|
Mannose Induces an Endonuclease Responsible for DNA Laddering in
Plant Cells
Joshua C. Stein1 and
Geneviève Hansen*
Novartis Agribusiness Biotechnology Research, 3054 Cornwallis Road,
Research Triangle Park, Durham, North Carolina 27709
The effect of D-mannose
(Man) on plant cells was studied in two different systems: Arabidopsis
roots and maize (Zea mays) suspension-cultured cells. In
both systems, exposure to D-Man was associated with a
subset of features characteristic of apoptosis, as assessed by
oligonucleosomal fragmentation and microscopy analysis. Furthermore,
D-Man induced the release of cytochrome c
from mitochondria. The specificity of D-Man was evaluated
by comparing the effects of diastereomers such as L-Man,
D-glucose, and D-galactose. Of these
treatments, only D-Man caused a reduction in final fresh weight with concomitant oligonucleosomal fragmentation. Man-induced DNA
laddering coincided with the activation of a DNase in maize cytosolic
extracts and with the appearance of single 35-kD band detected using an
in-gel DNase assay. The DNase activity was further confirmed by using
covalently closed circular plasmid DNA as a substrate. It appears that
D-Man, a safe and readily accessible compound, offers
remarkable features for the study of apoptosis in plant cells.
1
Present address: Cereon Genomics, 270 Albany
Street, Cambridge, MA 02139.
*
Corresponding author; e-mail
Genevieve.Hansen{at}NABRI.Novartis.com; fax 919-541-8585.
Plant Physiol. (1999) 121: 71-80
Copyright Clearance Center: 0032-0889/99/121//10
© 1999 American Society of Plant Physiologists
This article has been cited by other articles:
|
|
|
|
 
L. Zhang and D. Xing
Methyl Jasmonate Induces Production of Reactive Oxygen Species and Alterations in Mitochondrial Dynamics that Precede Photosynthetic Dysfunction and Subsequent Cell Death
Plant Cell Physiol.,
July 1, 2008;
49(7):
1092 - 1111.
[Abstract]
[Full Text]
[PDF]
|
|
|
|
|
|
|
A. K. Azad, T. Ishikawa, T. Ishikawa, Y. Sawa, and H. Shibata
Intracellular energy depletion triggers programmed cell death during petal senescence in tulip
J. Exp. Bot.,
May 31, 2008;
(2008)
ern066v1.
[Abstract]
[Full Text]
[PDF]
|
|
|
|
|
|
|
T. J. Reape, E. M. Molony, and P. F. McCabe
Programmed cell death in plants: distinguishing between different modes
J. Exp. Bot.,
February 5, 2008;
(2008)
erm258v1.
[Abstract]
[Full Text]
[PDF]
|
|
|
|
|
|
|
L. L. Torres and G. L. Salerno
A metabolic pathway leading to mannosylfructose biosynthesis in Agrobacterium tumefaciens uncovers a family of mannosyltransferases
PNAS,
September 4, 2007;
104(36):
14318 - 14323.
[Abstract]
[Full Text]
[PDF]
|
|
|
|
|
|
|
L. Lombardi, S. Casani, N. Ceccarelli, L. Galleschi, P. Picciarelli, and R. Lorenzi
Programmed cell death of the nucellus during Sechium edule Sw. seed development is associated with activation of caspase-like proteases
J. Exp. Bot.,
August 28, 2007;
(2007)
erm137v1.
[Abstract]
[Full Text]
[PDF]
|
|
|
|
|
|
|
R. A. Vacca, D. Valenti, A. Bobba, R. S. Merafina, S. Passarella, and E. Marra
Cytochrome c Is Released in a Reactive Oxygen Species-Dependent Manner and Is Degraded via Caspase-Like Proteases in Tobacco Bright-Yellow 2 Cells en Route to Heat Shock-Induced Cell Death
Plant Physiology,
May 1, 2006;
141(1):
208 - 219.
[Abstract]
[Full Text]
[PDF]
|
|
|
|
|
|
|
J. M. R. Faria, J. Buitink, A. A. M. van Lammeren, and H. W. M. Hilhorst
Changes in DNA and microtubules during loss and re-establishment of desiccation tolerance in germinating Medicago truncatula seeds
J. Exp. Bot.,
August 1, 2005;
56(418):
2119 - 2130.
[Abstract]
[Full Text]
[PDF]
|
|
|
|
|
|
|
E. A. A. da Silva, P. E. Toorop, J. Nijsse, J. D. Bewley, and H. W. M. Hilhorst
Exogenous gibberellins inhibit coffee (Coffea arabica cv. Rubi) seed germination and cause cell death in the embryo
J. Exp. Bot.,
March 1, 2005;
56(413):
1029 - 1038.
[Abstract]
[Full Text]
[PDF]
|
|
|
|
|
|
|
G. C. Pagnussat, H.-J. Yu, Q. A. Ngo, S. Rajani, S. Mayalagu, C. S. Johnson, A. Capron, L.-F. Xie, D. Ye, and V. Sundaresan
Genetic and molecular identification of genes required for female gametophyte development and function in Arabidopsis
Development,
February 1, 2005;
132(3):
603 - 614.
[Abstract]
[Full Text]
[PDF]
|
|
|
|
|
|
|
S. Chen and M. B. Dickman
Bcl-2 family members localize to tobacco chloroplasts and inhibit programmed cell death induced by chloroplast-targeted herbicides
J. Exp. Bot.,
December 1, 2004;
55(408):
2617 - 2623.
[Abstract]
[Full Text]
[PDF]
|
|
|
|
|
|
|
S. W. Yang, S. K. Kim, and W. T. Kim
Perturbation of NgTRF1 Expression Induces Apoptosis-Like Cell Death in Tobacco BY-2 Cells and Implicates NgTRF1 in the Control of Telomere Length and Stability
PLANT CELL,
December 1, 2004;
16(12):
3370 - 3385.
[Abstract]
[Full Text]
[PDF]
|
|
|
|
|
|
|
J. Ito and H. Fukuda
ZEN1 Is a Key Enzyme in the Degradation of Nuclear DNA during Programmed Cell Death of Tracheary Elements
PLANT CELL,
December 1, 2002;
14(12):
3201 - 3211.
[Abstract]
[Full Text]
[PDF]
|
|
|
|
|
|
|
E. VIROLAINEN, O. BLOKHINA, and K. FAGERSTEDT
Ca2+-induced High Amplitude Swelling and Cytochrome c Release From Wheat (Triticum aestivum L.) Mitochondria Under Anoxic Stress
Ann. Bot.,
October 1, 2002;
90(4):
509 - 516.
[Abstract]
[Full Text]
[PDF]
|
|
|
|
|
|
|
C. A. Christensen, S. W. Gorsich, R. H. Brown, L. G. Jones, J. Brown, J. M. Shaw, and G. N. Drews
Mitochondrial GFA2 Is Required for Synergid Cell Death in Arabidopsis
PLANT CELL,
September 1, 2002;
14(9):
2215 - 2232.
[Abstract]
[Full Text]
[PDF]
|
|
|
|
|
|
|
F. Rolland, B. Moore, and J. Sheen
Sugar Sensing and Signaling in Plants
PLANT CELL,
May 1, 2002;
14(90001):
S185 - 205.
[Full Text]
[PDF]
|
|
|
|
|
|
|
S.-B. Ning, L. Wang, and Y.-C. Song
Identification of programmed cell death in situ in individual plant cells in vivo using a chromosome preparation technique
J. Exp. Bot.,
April 1, 2002;
53(369):
651 - 658.
[Abstract]
[Full Text]
[PDF]
|
|
|
|
|
|
|
J. Balk and C. J. Leaver
The PET1-CMS Mitochondrial Mutation in Sunflower Is Associated with Premature Programmed Cell Death and Cytochrome c Release
PLANT CELL,
August 1, 2001;
13(8):
1803 - 1818.
[Abstract]
[Full Text]
[PDF]
|
|
|
|
|
|
|
R. Brouquisse, A. Evrard, D. Rolin, P. Raymond, and C. Roby
Regulation of Protein Degradation and Protease Expression by Mannose in Maize Root Tips. Pi Sequestration by Mannose May Hinder the Study of Its Signaling Properties
Plant Physiology,
March 1, 2001;
125(3):
1485 - 1498.
[Abstract]
[Full Text]
|
|
|
|
|
|
|
W. Lukowitz, T. C. Nickle, D. W. Meinke, R. L. Last, P. L. Conklin, and C. R. Somerville
Arabidopsis cyt1 mutants are deficient in a mannose-1-phosphate guanylyltransferase and point to a requirement of N-linked glycosylation for cellulose biosynthesis
PNAS,
February 15, 2001;
(2001)
51625798.
[Abstract]
[Full Text]
|
|
|
|
|
|
|
K. Obara, H. Kuriyama, and H. Fukuda
Direct Evidence of Active and Rapid Nuclear Degradation Triggered by Vacuole Rupture during Programmed Cell Death in Zinnia
Plant Physiology,
February 1, 2001;
125(2):
615 - 626.
[Abstract]
[Full Text]
|
|
|
|
|
|
|
P. Xu and M. J. Roossinck
Cucumber Mosaic Virus D Satellite RNA-Induced Programmed Cell Death in Tomato
PLANT CELL,
July 1, 2000;
12(7):
1079 - 1092.
[Abstract]
[Full Text]
|
|
|
|
|
|
|
Y. Xu and M. R. Hanson
Programmed Cell Death during Pollination-Induced Petal Senescence in Petunia
Plant Physiology,
April 1, 2000;
122(4):
1323 - 1334.
[Abstract]
[Full Text]
|
|
|
|
|
|
|
S. Arpagaus, A. Rawyler, and R. Braendle
Occurrence and Characteristics of the Mitochondrial Permeability Transition in Plants
J. Biol. Chem.,
January 11, 2002;
277(3):
1780 - 1787.
[Abstract]
[Full Text]
[PDF]
|
|
|
|
|
|
|
W. Lukowitz, T. C. Nickle, D. W. Meinke, R. L. Last, P. L. Conklin, and C. R. Somerville
Arabidopsis cyt1 mutants are deficient in a mannose-1-phosphate guanylyltransferase and point to a requirement of N-linked glycosylation for cellulose biosynthesis
PNAS,
February 27, 2001;
98(5):
2262 - 2267.
[Abstract]
[Full Text]
[PDF]
|
|
|
|
|
