Plant Physiol. Journal of Pharmacology and Experimental Therapeutics
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Differences in Spatial Expression between 14-3-3 Isoforms in Germinating Barley Embryos1

Christa Testerink*, René M. van der Meulen, Berry J. Oppedijk, Albertus H. de Boer, Sjoukje Heimovaara-Dijkstra, Jan W. Kijne, and Mei Wang

Center for Phytotechnology, Leiden University/The Netherlands Organization for Applied Scientific Research (RUL/TNO), TNO Department of Plant Biotechnology, Wassenaarseweg 64, 2333 AL Leiden, The Netherlands (C.T., R.M.v.d.M., B.J.O., S.H.-D., M.W.); Department of Genetics, Section of Plant Physiology, Vrije Universiteit, de Boelelaan 1087, 1081 HV Amsterdam, The Netherlands (A.H.d.B.); and Center for Phytotechnology, RUL/TNO, Institute of Molecular Plant Sciences, Leiden University, Wassenaarseweg 64, 2333 AL Leiden, The Netherlands (J.W.K.)

The family of 14-3-3 proteins is ubiquitous in eukaryotes and has been shown to exert an array of functions. We were interested in the possible role of 14-3-3 proteins in seed germination. Therefore, we studied the expression of 14-3-3 mRNA and protein in barley (Hordeum distichum L.) embryos during germination. With the use of specific cDNA probes and antibodies, we could detect individual expression of three 14-3-3 isoforms, 14-3-3A, 14-3-3B, and 14-3-3C. Each homolog was found to be expressed in barley embryos. Whereas protein levels of all three isoforms were constant during germination, mRNA expression was found to be induced upon imbibition of the grains. The induction of 14-3-3A gene expression during germination was different from that of 14-3-3B and 14-3-3C. In situ immunolocalization analysis showed similar spatial expression for 14-3-3A and 14-3-3B, while 14-3-3C expression was markedly different. Whereas 14-3-3A and 14-3-3B were expressed throughout the embryo, 14-3-3C expression was tissue specific, with the strongest expression observed in the scutellum and the L2 layer of the shoot apical meristem. These results show that 14-3-3 homologs are differently regulated in barley embryos, and provide a first step in acquiring more knowledge about the role of 14-3-3 proteins in the germination process.

1   This work was partially supported by European Community program no. PL962275 and by the Dutch Technology Foundation project no. 805.22.765.
*   Corresponding author; e-mail testerink{at}rulbim.leidenuniv.nl; fax 31-71-5274863.

Plant Physiol. (1999) 121: 81-88
Copyright Clearance Center:   0032-0889/99/121//08
© 1999 American Society of Plant Physiologists




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