|
Plant Physiol, October 1999, Vol. 121, pp. 383-390
The mur4 Mutant of Arabidopsis Is Partially Defective
in the de Novo Synthesis of Uridine Diphospho
L-Arabinose1
Emilie G.
Burget and
Wolf-Dieter
Reiter*
Department of Molecular and Cell Biology, University of
Connecticut, Storrs, Connecticut 06269
To obtain information on the
synthesis and function of arabinosylated glycans, the
mur4 mutant of Arabidopsis was characterized. This
mutation leads to a 50% reduction in the monosaccharide
L-arabinose in most organs and affects arabinose-containing
pectic cell wall polysaccharides and arabinogalactan proteins. Feeding
L-arabinose to mur4 plants restores the cell
wall composition to wild-type levels, suggesting a partial defect in
the de novo synthesis of UDP-L-arabinose, the activated
sugar used by arabinosyltransferases. The defect was traced to the
conversion of UDP-D-xylose to UDP-L-arabinose in the microsome fraction of leaf material, indicating that
mur4 plants are defective in a membrane-bound
UDP-D-xylose 4-epimerase.
1
This work was supported by the U.S. Department
of Energy.
*
Corresponding author; e-mail wdreiter{at}uconnvm.uconn.edu; fax
860-486-4331.
© 1999 American Society of Plant Physiologists
This article has been cited by other articles:
|
|
|
|
 
R. A. Chavez Montes, P. Ranocha, Y. Martinez, Z. Minic, L. Jouanin, M. Marquis, L. Saulnier, L. M. Fulton, C. S. Cobbett, F. Bitton, et al.
Cell Wall Modifications in Arabidopsis Plants with Altered {alpha}-L-Arabinofuranosidase Activity
Plant Physiology,
May 1, 2008;
147(1):
63 - 77.
[Abstract]
[Full Text]
[PDF]
|
|
|
|
|
|
|
J.-C. Leple, R. Dauwe, K. Morreel, V. Storme, C. Lapierre, B. Pollet, A. Naumann, K.-Y. Kang, H. Kim, K. Ruel, et al.
Downregulation of Cinnamoyl-Coenzyme A Reductase in Poplar: Multiple-Level Phenotyping Reveals Effects on Cell Wall Polymer Metabolism and Structure
PLANT CELL,
November 1, 2007;
19(11):
3669 - 3691.
[Abstract]
[Full Text]
[PDF]
|
|
|
|
|
|
|
Y. Li, C. Smith, F. Corke, L. Zheng, Z. Merali, P. Ryden, P. Derbyshire, K. Waldron, and M. W. Bevan
Signaling from an Altered Cell Wall to the Nucleus Mediates Sugar-Responsive Growth and Development in Arabidopsis thaliana
PLANT CELL,
August 1, 2007;
19(8):
2500 - 2515.
[Abstract]
[Full Text]
[PDF]
|
|
|
|
|
|
|
G. Mouille, H. Witucka-Wall, M.-P. Bruyant, O. Loudet, S. Pelletier, C. Rihouey, O. Lerouxel, P. Lerouge, H. Hofte, and M. Pauly
Quantitative Trait Loci Analysis of Primary Cell Wall Composition in Arabidopsis
Plant Physiology,
July 1, 2006;
141(3):
1035 - 1044.
[Abstract]
[Full Text]
[PDF]
|
|
|
|
|
|
|
G.-E. Stenvik, M. A. Butenko, B. R. Urbanowicz, J. K.C. Rose, and R. B. Aalen
Overexpression of INFLORESCENCE DEFICIENT IN ABSCISSION Activates Cell Separation in Vestigial Abscission Zones in Arabidopsis
PLANT CELL,
June 1, 2006;
18(6):
1467 - 1476.
[Abstract]
[Full Text]
[PDF]
|
|
|
|
|
|
|
Q. Zhang, N. Shirley, J. Lahnstein, and G. B. Fincher
Characterization and Expression Patterns of UDP-D-Glucuronate Decarboxylase Genes in Barley
Plant Physiology,
May 1, 2005;
138(1):
131 - 141.
[Abstract]
[Full Text]
[PDF]
|
|
|
|
|
|
|
T. Kotake, D. Yamaguchi, H. Ohzono, S. Hojo, S. Kaneko, H.-k. Ishida, and Y. Tsumuraya
UDP-sugar Pyrophosphorylase with Broad Substrate Specificity Toward Various Monosaccharide 1-Phosphates from Pea Sprouts
J. Biol. Chem.,
October 29, 2004;
279(44):
45728 - 45736.
[Abstract]
[Full Text]
[PDF]
|
|
|
|
|
|
|
E. G. Burget, R. Verma, M. Molhoj, and W.-D. Reiter
The Biosynthesis of L-Arabinose in Plants: Molecular Cloning and Characterization of a Golgi-Localized UDP-D-Xylose 4-Epimerase Encoded by the MUR4 Gene of Arabidopsis
PLANT CELL,
February 1, 2003;
15(2):
523 - 531.
[Abstract]
[Full Text]
[PDF]
|
|
|
|
|
|
|
M. Kobayashi, H. Nakagawa, I. Suda, I. Miyagawa, and T. Matoh
Purification and cDNA Cloning of UDP-D-Glucuronate Carboxy-lyase (UDP-D-xylose Synthase) from Pea Seedlings
Plant Cell Physiol.,
November 15, 2002;
43(11):
1259 - 1265.
[Abstract]
[Full Text]
[PDF]
|
|
|
|
|
|
|
M. Skjot, M. Pauly, M. S. Bush, B. Borkhardt, M. C. McCann, and P. Ulvskov
Direct Interference with Rhamnogalacturonan I Biosynthesis in Golgi Vesicles
Plant Physiology,
May 1, 2002;
129(1):
95 - 102.
[Abstract]
[Full Text]
[PDF]
|
|
|
|
|
|
|
G. F. Vanzin, M. Madson, N. C. Carpita, N. V. Raikhel, K. Keegstra, and W.-D. Reiter
The mur2 mutant of Arabidopsis thaliana lacks fucosylated xyloglucan because of a lesion in fucosyltransferase AtFUT1
PNAS,
February 14, 2002;
(2002)
52450699.
[Abstract]
[Full Text]
[PDF]
|
|
|
|
|
|
|
T. L. Western, J. Burn, W. L. Tan, D. J. Skinner, L. Martin-McCaffrey, B. A. Moffatt, and G. W. Haughn
Isolation and Characterization of Mutants Defective in Seed Coat Mucilage Secretory Cell Development in Arabidopsis
Plant Physiology,
November 1, 2001;
127(3):
998 - 1011.
[Abstract]
[Full Text]
[PDF]
|
|
|
|
|
|
|
G. F. Vanzin, M. Madson, N. C. Carpita, N. V. Raikhel, K. Keegstra, and W.-D. Reiter
The mur2 mutant of Arabidopsis thaliana lacks fucosylated xyloglucan because of a lesion in fucosyltransferase AtFUT1
PNAS,
March 5, 2002;
99(5):
3340 - 3345.
[Abstract]
[Full Text]
[PDF]
|
|
|
|
|
