|
Plant Physiol, October 1999, Vol. 121, pp. 399-410
The Starch-Debranching Enzymes Isoamylase and Pullulanase Are
Both Involved in Amylopectin Biosynthesis in Rice
Endosperm1
Akiko
Kubo,
Naoko
Fujita,2
Kyuya
Harada,
Toshiaki
Matsuda,
Hikaru
Satoh, and
Yasunori
Nakamura*
National Institute of Agrobiological Resources, Kannondai, Tsukuba,
Ibaraki 305-8602, Japan (A.K., N.F., Y.N.); Department of
Horticulture, Chiba University, Matsudo, Chiba 271-8510, Japan (A.K.,
K.H.); School of Agriculture, Ibaraki University, Ami, Tsuchiura,
Ibaraki 300-0393, Japan (T.M.); and Faculty of Agriculture, Kyushu
University, Hakozaki, Higashi-ku, Fukuoka 812-8581, Japan (H.S.)
The activities of the two types of
starch debranching enzymes, isoamylase and pullulanase, were greatly
reduced in endosperms of allelic sugary-1 mutants of
rice (Oryza sativa), with the decrease more pronounced
for isoamylase than for pullulanase. However, the decrease in
isoamylase activity was not related to the magnitude of the
sugary phenotype (the proportion of the phytoglycogen
region of the endosperm), as observed with pullulanase. In the
moderately mutated line EM-5, the pullulanase activity was markedly
lower in the phytoglycogen region than in the starch region, and
isoamylase activity was extremely low or completely lost in the whole
endosperm tissue. These results suggest that both debranching enzymes
are involved in amylopectin biosynthesis in rice endosperm. We presume that isoamylase plays a predominant role in amylopectin synthesis, but
pullulanase is also essential or can compensate for the role of
isoamylase in the construction of the amylopectin multiple-cluster structure. It is highly possible that isoamylase was modified in some
sugary-1 mutants such as EM-273 and EM-5, since it was present in significant and trace amounts, respectively, in these mutants but was apparently inactive. The results show that the Sugary-1 gene encodes the isoamylase gene of the rice genome.
1
This work was supported by grants from Special
Coordination Funds for Promoting Science and Technology, The
Enhancement of Center-of-Excellence Program, Japan, and from the
Ministry of Agriculture, Forestry and Fisheries, Japan.
2
Present address: Department of Bio-Production
Science, Akita Prefectural University, Akita, Akita 010-0146, Japan.
*
Corresponding author; e-mail nakayn{at}abr.affrc.go.jp; fax
81-298-38-8347.
© 1999 American Society of Plant Physiologists
This article has been cited by other articles:
|
|
|
|
 
J.-H. Shim, J.-T. Park, J.-S. Hong, K. W. Kim, M.-J. Kim, J.-H. Auh, Y.-W. Kim, C.-S. Park, W. Boos, J.-W. Kim, et al.
Role of Maltogenic Amylase and Pullulanase in Maltodextrin and Glycogen Metabolism of Bacillus subtilis 168
J. Bacteriol.,
August 1, 2009;
191(15):
4835 - 4844.
[Abstract]
[Full Text]
[PDF]
|
|
|
|
|
|
|
T. Sawada, P. B. Francisco Jr, S. Aihara, Y. Utsumi, M. Yoshida, Y. Oyama, M. Tsuzuki, H. Satoh, and Y. Nakamura
Chlorella Starch Branching Enzyme II (BEII) Can Complement the Function of BEIIb in Rice Endosperm
Plant Cell Physiol.,
June 1, 2009;
50(6):
1062 - 1074.
[Abstract]
[Full Text]
[PDF]
|
|
|
|
|
|
|
N. Fujita, Y. Toyosawa, Y. Utsumi, T. Higuchi, I. Hanashiro, A. Ikegami, S. Akuzawa, M. Yoshida, A. Mori, K. Inomata, et al.
Characterization of pullulanase (PUL)-deficient mutants of rice (Oryza sativa L.) and the function of PUL on starch biosynthesis in the developing rice endosperm
J. Exp. Bot.,
March 1, 2009;
60(3):
1009 - 1023.
[Abstract]
[Full Text]
[PDF]
|
|
|
|
|
|
|
S. Streb, T. Delatte, M. Umhang, S. Eicke, M. Schorderet, D. Reinhardt, and S. C. Zeeman
Starch Granule Biosynthesis in Arabidopsis Is Abolished by Removal of All Debranching Enzymes but Restored by the Subsequent Removal of an Endoamylase
PLANT CELL,
December 1, 2008;
20(12):
3448 - 3466.
[Abstract]
[Full Text]
[PDF]
|
|
|
|
|
|
|
F. Wattebled, V. Planchot, Y. Dong, N. Szydlowski, B. Pontoire, A. Devin, S. Ball, and C. D'Hulst
Further Evidence for the Mandatory Nature of Polysaccharide Debranching for the Aggregation of Semicrystalline Starch and for Overlapping Functions of Debranching Enzymes in Arabidopsis Leaves
Plant Physiology,
November 1, 2008;
148(3):
1309 - 1323.
[Abstract]
[Full Text]
[PDF]
|
|
|
|
|
|
|
T. Shimonaga, M. Konishi, Y. Oyama, S. Fujiwara, A. Satoh, N. Fujita, C. Colleoni, A. Buleon, J.-L. Putaux, S. G. Ball, et al.
Variation in Storage {alpha}-Glucans of the Porphyridiales (Rhodophyta)
Plant Cell Physiol.,
January 1, 2008;
49(1):
103 - 116.
[Abstract]
[Full Text]
[PDF]
|
|
|
|
|
|
|
L. Li, H. Ilarslan, M. G. James, A. M. Myers, and E. S. Wurtele
Genome wide co-expression among the starch debranching enzyme genes AtISA1, AtISA2, and AtISA3 in Arabidopsis thaliana
J. Exp. Bot.,
September 20, 2007;
(2007)
erm180v1.
[Abstract]
[Full Text]
[PDF]
|
|
|
|
|
|
|
N. Fujita, M. Yoshida, T. Kondo, K. Saito, Y. Utsumi, T. Tokunaga, A. Nishi, H. Satoh, J.-H. Park, J.-L. Jane, et al.
Characterization of SSIIIa-Deficient Mutants of Rice: The Function of SSIIIa and Pleiotropic Effects by SSIIIa Deficiency in the Rice Endosperm
Plant Physiology,
August 1, 2007;
144(4):
2009 - 2023.
[Abstract]
[Full Text]
[PDF]
|
|
|
|
|
|
|
H. Yamakawa, T. Hirose, M. Kuroda, and T. Yamaguchi
Comprehensive Expression Profiling of Rice Grain Filling-Related Genes under High Temperature Using DNA Microarray
Plant Physiology,
May 1, 2007;
144(1):
258 - 277.
[Abstract]
[Full Text]
[PDF]
|
|
|
|
|
|
|
T. Ohdan, P. B. Francisco Jr, T. Sawada, T. Hirose, T. Terao, H. Satoh, and Y. Nakamura
Expression profiling of genes involved in starch synthesis in sink and source organs of rice
J. Exp. Bot.,
December 1, 2005;
56(422):
3229 - 3244.
[Abstract]
[Full Text]
[PDF]
|
|
|
|
|
|
|
F. Wattebled, Y. Dong, S. Dumez, D. Delvalle, V. Planchot, P. Berbezy, D. Vyas, P. Colonna, M. Chatterjee, S. Ball, et al.
Mutants of Arabidopsis Lacking a Chloroplastic Isoamylase Accumulate Phytoglycogen and an Abnormal Form of Amylopectin
Plant Physiology,
May 1, 2005;
138(1):
184 - 195.
[Abstract]
[Full Text]
[PDF]
|
|
|
|
|
|
|
G. Barbier, C. Oesterhelt, M. D. Larson, R. G. Halgren, C. Wilkerson, R. M. Garavito, C. Benning, and A. P.M. Weber
Comparative Genomics of Two Closely Related Unicellular Thermo-Acidophilic Red Algae, Galdieria sulphuraria and Cyanidioschyzon merolae, Reveals the Molecular Basis of the Metabolic Flexibility of Galdieria sulphuraria and Significant Differences in Carbohydrate Metabolism of Both Algae
Plant Physiology,
February 1, 2005;
137(2):
460 - 474.
[Abstract]
[Full Text]
[PDF]
|
|
|
|
|
|
|
A. Kubo, S. Rahman, Y. Utsumi, Z. Li, Y. Mukai, M. Yamamoto, M. Ugaki, K. Harada, H. Satoh, C. Konik-Rose, et al.
Complementation of sugary-1 Phenotype in Rice Endosperm with the Wheat Isoamylase1 Gene Supports a Direct Role for Isoamylase1 in Amylopectin Biosynthesis
Plant Physiology,
January 1, 2005;
137(1):
43 - 56.
[Abstract]
[Full Text]
[PDF]
|
|
|
|
|
|
|
R. Bustos, B. Fahy, C. M. Hylton, R. Seale, N. M. Nebane, A. Edwards, C. Martin, and A. M. Smith
Starch granule initiation is controlled by a heteromultimeric isoamylase in potato tubers
PNAS,
February 17, 2004;
101(7):
2215 - 2220.
[Abstract]
[Full Text]
[PDF]
|
|
|
|
|
|
|
N. Fujita, A. Kubo, D.-S. Suh, K.-S. Wong, J.-L. Jane, K. Ozawa, F. Takaiwa, Y. Inaba, and Y. Nakamura
Antisense Inhibition of Isoamylase Alters the Structure of Amylopectin and the Physicochemical Properties of Starch in Rice Endosperm
Plant Cell Physiol.,
June 15, 2003;
44(6):
607 - 618.
[Abstract]
[Full Text]
[PDF]
|
|
|
|
|
|
|
J. R. Dinges, C. Colleoni, M. G. James, and A. M. Myers
Mutational Analysis of the Pullulanase-Type Debranching Enzyme of Maize Indicates Multiple Functions in Starch Metabolism
PLANT CELL,
March 1, 2003;
15(3):
666 - 680.
[Abstract]
[Full Text]
[PDF]
|
|
|
|
|
|
|
H. Hussain, A. Mant, R. Seale, S. Zeeman, E. Hinchliffe, A. Edwards, C. Hylton, S. Bornemann, A. M. Smith, C. Martin, et al.
Three Isoforms of Isoamylase Contribute Different Catalytic Properties for the Debranching of Potato Glucans
PLANT CELL,
January 1, 2003;
15(1):
133 - 149.
[Abstract]
[Full Text]
[PDF]
|
|
|
|
|
|
|
Y. Nakamura
Towards a Better Understanding of the Metabolic System for Amylopectin Biosynthesis in Plants: Rice Endosperm as a Model Tissue
Plant Cell Physiol.,
July 15, 2002;
43(7):
718 - 725.
[Abstract]
[Full Text]
[PDF]
|
|
|
|
|
|
|
T. H. Nielsen, L. Baunsgaard, and A. Blennow
Intermediary Glucan Structures Formed during Starch Granule Biosynthesis Are Enriched in Short Side Chains, a Dynamic Pulse Labeling Approach
J. Biol. Chem.,
May 31, 2002;
277(23):
20249 - 20255.
[Abstract]
[Full Text]
[PDF]
|
|
|
|
|
|
|
D. Dauvillée, C. Colleoni, G. Mouille, A. Buléon, D. J. Gallant, B. Bouchet, M. K. Morell, C. d'Hulst, A. M. Myers, and S. G. Ball
Two Loci Control Phytoglycogen Production in the Monocellular Green Alga Chlamydomonas reinhardtii
Plant Physiology,
April 1, 2001;
125(4):
1710 - 1722.
[Abstract]
[Full Text]
|
|
|
|
|
|
|
D. Dauvillée, C. Colleoni, G. Mouille, M. K. Morell, C. d'Hulst, F. Wattebled, L. Liénard, D. Delvallé, J.-P. Ral, A. M. Myers, et al.
Biochemical Characterization of Wild-Type and Mutant Isoamylases of Chlamydomonas reinhardtii Supports a Function of the Multimeric Enzyme Organization in Amylopectin Maturation
Plant Physiology,
April 1, 2001;
125(4):
1723 - 1731.
[Abstract]
[Full Text]
|
|
|
|
|
|
|
J. R. Dinges, C. Colleoni, A. M. Myers, and M. G. James
Molecular Structure of Three Mutations at the Maize sugary1 Locus and Their Allele-Specific Phenotypic Effects
Plant Physiology,
March 1, 2001;
125(3):
1406 - 1418.
[Abstract]
[Full Text]
|
|
|
|
|
|
|
A. M. Myers, M. K. Morell, M. G. James, and S. G. Ball
Recent Progress toward Understanding Biosynthesis of the Amylopectin Crystal
Plant Physiology,
April 1, 2000;
122(4):
989 - 998.
[Full Text]
|
|
|
|
|
