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Plant Physiol, October 1999, Vol. 121, pp. 429-436
Arabidopsis Alcohol Dehydrogenase Expression in Both Shoots
and Roots Is Conditioned by Root Growth Environment1
Hwa-Jee
Chung and
Robert J.
Ferl*
Program in Plant Molecular and Cellular Biology, Department of
Horticultural Sciences, University of Florida, Gainesville, Florida
32611
It is widely accepted that the
Arabidopsis Adh (alcohol dehydrogenase) gene is
constitutively expressed at low levels in the roots of young plants
grown on agar media, and that the expression level is greatly induced
by anoxic or hypoxic stresses. We questioned whether the agar medium
itself created an anaerobic environment for the roots upon their
growing into the gel.  -Glucuronidase (GUS) expression driven by the
Adh promoter was examined by growing transgenic
Arabidopsis plants in different growing systems. Whereas roots grown on
horizontal-positioned plates showed high Adh/GUS expression levels,
roots from vertical-positioned plates had no Adh/GUS expression.
Additional results indicate that growth on vertical plates closely
mimics the Adh/GUS expression observed for soil-grown seedlings, and
that growth on horizontal plates results in induction of high Adh/GUS
expression that is consistent with hypoxic or anoxic conditions within
the agar of the root zone. Adh/GUS expression in the shoot apex is also
highly induced by root penetration of the agar medium. This induction
of Adh/GUS in shoot apex and roots is due, at least in part, to
mechanisms involving Ca2+ signal transduction.
1
This research was supported by the National
Aeronautics and Space Agency (grant no. NAG10-0145 to R.J.F.). This
manuscript is journal series no. R-06984 of the Florida
Agricultural Experiment Station.
*
Corresponding author; e-mail robferl{at}ufl.edu; fax
352-392-4072.
© 1999 American Society of Plant Physiologists
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