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Plant Physiol, October 1999, Vol. 121, pp. 571-578
Dynamic Properties of Endogenous Phytochrome A in
Arabidopsis Seedlings1
Lars
Hennig,
Claudia
Büche,
Klaus
Eichenberg, and
Eberhard
Schäfer*
Institut für Biologie II, Universität Freiburg,
Schänzlestrasse 1, 79104 Freiburg, Germany
The dynamic behavior of phytochrome A
(phyA) in seedlings of the model plant Arabidopsis was examined by in
vivo spectroscopy and by western and northern blotting. Rapid
accumulation of phyA was observed, reaching a steady state after 3 d. Both red and far-red light initiated a rapid destruction of the
far-red-light-absorbing form of phytochrome (Pfr); the apparent
half-life was only 4-fold longer in far-red than in red light.
Furthermore, the Pfr-induced destruction of the red-light-absorbing
form of phytochrome (Pr) of phyA occurred in darkness with a rate
identical to that of Pfr destruction. A 2-fold decrease in mRNA
abundance was observed after irradiation, irrespective of the applied
light quality. However, reaccumulation occurred rapidly after far-red
but slowly after red irradiation, indicating different modes of
regulation of phytochrome expression after light-dark transitions
depending on the light quality of the preceding irradiation. The
wavelength dependency of the destruction rates was distinct from that
of mustard, a close relative of Arabidopsis, and was explained on the
basis of Pfr-induced Pr destruction and a simple kinetic two-step model. No dark reversion was detectable in the destruction kinetics after a red pulse. From these data we conclude that Arabidopsis phyA
differs significantly in several aspects from other dicot phytochromes.
1
This work was supported by the Deutsch
Forschungsgemeinschaft (fellowship to L.H. and grant to E.S.) and by
Evangelisches Studienwerk Villigst (fellowship to K.E.).
*
Corresponding author; e-mail schaegen{at}ruf.uni-freiburg.de; fax
49-761-203-2629.
© 1999 American Society of Plant Physiologists
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