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Plant Physiol, October 1999, Vol. 121, pp. 579-588

Directed Mutation of the Rubisco Large Subunit of Tobacco Influences Photorespiration and Growth1

Spencer M. Whitney, Susanne von Caemmerer, Graham S. Hudson,2 and T. John Andrews*

Molecular Plant Physiology, Research School of Biological Sciences, Australian National University, P.O. Box 475, Canberra, Australian Capital Territory 2601, Australia

The gene for the large subunit of Rubisco was specifically mutated by transforming the chloroplast genome of tobacco (Nicotiana tabacum). Codon 335 was altered to encode valine instead of leucine. The resulting mutant plants could not grow without atmospheric CO2 enrichment. In 0.3% (v/v) CO2, the mutant and wild-type plants produced similar amounts of Rubisco but the extent of carbamylation was nearly twice as great in the mutants. The mutant enzyme's substrate-saturated CO2-fixing rate and its ability to distinguish between CO2 and O2 as substrates were both reduced to 25% of the wild type's values. Estimates of these parameters obtained from kinetic assays with the purified mutant enzyme were the same as those inferred from measurements of photosynthetic gas exchange with leaves of mutant plants. The Michaelis constants for CO2, O2, and ribulose-1,5-bisphosphate were reduced and the mutation enhanced oxygenase activity at limiting O2 concentrations. Consistent with the reduced CO2 fixation rate at saturating CO2, the mutant plants grew slower than the wild type but they eventually flowered and reproduced apparently normally. The mutation and its associated phenotype were inherited maternally. The chloroplast-transformation strategy surmounts previous obstacles to mutagenesis of higher-plant Rubisco and allows the consequences for leaf photosynthesis to be assessed.

1 This work was supported by the Australian National University's Centre for Molecular Structure and Function.

2 Present address: 12 Jansz Crescent, Griffith, ACT 2603, Australia.

* Corresponding author; e-mail john.andrews{at}anu.edu.au; fax 61-2-6249-5075.

© 1999 American Society of Plant Physiologists


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