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Plant Physiol, October 1999, Vol. 121, pp. 647-656
Rates of Sugar Uptake by Guard Cell Protoplasts of
Pisum sativum L. Related to the Solute Requirement for
Stomatal Opening1
Gerhard
Ritte,2
Johanna
Rosenfeld,
Kerstin
Rohrig, and
Klaus
Raschke*
Albrecht-von-Haller-Institut für Pflanzenwissenschaften,
Universität Göttingen, Untere Karspüle 2, 37073 Göttingen, Germany
We
wished to determine whether the capacity of the sugar uptake mechanisms
of guard cells of the Argenteum mutant of pea (Pisum sativum L.) sufficed to support a concurrent stomatal opening movement. Sugar uptake by guard cell protoplasts was determined by
silicone-oil-filtering centrifugation. The protoplasts took up
[14C]glucose, [14C]fructose, and
[14C]sucrose (Suc), apparently in symport with protons.
Mannose, galactose, and fructose competed with Glc for transport by a
presumed hexose carrier. The uptake of Glc saturated with a
Km of 0.12 mM and a
Vmax of 19 fmol cell 1
h1. At external concentrations <1 mM, the
uptake of Suc was slower than that of Glc. It exhibited a saturating
component with a Km varying between 0.25 and
0.8 mM and a Vmax between 1 and
10 fmol cell1 h1, and at external
concentrations >1 mM, a non-saturating component. At
apoplastic sugar concentrations below 4 mM, sugar import
was estimated to be mainly in the form of hexoses and too slow to support a simultaneous stomatal opening movement. If, however, during
times of high photosynthesis and transpiration, the apoplastic Suc
concentration rose and entered the range of non-saturating import,
absorbed Suc could replace potassium malate as the osmoticum for the
maintenance of stomatal opening.
1
This work was supported grants to K.R. by the
Deutsche Forschungsgemeinschaft.
2
Present address: Institut für Biochemie
und Molekulare Physiologie, Universität Potsdam, Maulbeerallee 2, 14469 Potsdam, Germany.
*
Corresponding author; e-mail kraschk{at}gwdg.de; fax
49-551-397823.
© 1999 American Society of Plant Physiologists
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