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Plant Physiol, November 1999, Vol. 121, pp. 763-774
Loss of Tonoplast Integrity Programmed in Tracheary Element
Differentiation1
Hideo
Kuriyama*
Department of Biological Sciences, Graduate School of Science,
University of Tokyo, Hongo, Tokyo 113, Japan
A tracheary element (TE) is a typical
example of a cell type that undergoes programmed cell death in the
developmental processes of vascular plants. The loss of the selective
permeability of the tonoplast, which corresponds to tonoplast
disintegration, occurred after the cells commenced secondary wall
thickening and played a pivotal role in the programmed cell death of
TEs in a zinnia (Zinnia elegans L.) cell culture. A
search for events specifically associated with the TE vacuole provided
an important clue to the understanding of the cell death mechanism. The
transport of fluorescein, a fluorescent organic anion, across the
tonoplast declined drastically in differentiating TEs. The capacity of
the vacuole to accumulate the probe was also impaired. Treatment with
probenecid, an inhibitor of organic anion transport, caused rapid cell
death of TEs and led to the ultimate disruption of the vacuole even in
other types of cultured cells. These changes in vacuolar properties
during TE development were suppressed by cycloheximide. Specific mRNA accumulation in cells cultured in a TE differentiation-inductive condition was abolished by probenecid. These results suggest that a
change in vacuolar membrane permeability promotes programmed cell death
in TEs.
1
This work was supported by grants from the
Ministry of Education, Science and Culture of Japan and the Japan
Society for the Promotion of Science.
*
E-mail kuriyama{at}jade.dti.ne.jp or
kuriyama{at}biol.s.u-tokyo.ac.jp; fax 81-3-3812-4929.
© 1999 American Society of Plant Physiologists
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