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Plant Physiol, November 1999, Vol. 121, pp. 783-791

Regulation of Gibberellin 20-Oxidase and Gibberellin 3beta -Hydroxylase Transcript Accumulation during De-Etiolation of Pea Seedlings1

Tahar Ait-Ali,2* Shannon Frances, James L. Weller, James B. Reid, Richard E. Kendrick, and Yuji Kamiya

Laboratory for Plant Hormone Function (T.A.-A., Y.K.) and Laboratory for Photoperception and Signal Transduction (S.F., R.E.K.), Frontier Research Program, The Institute of Physical and Chemical Research (RIKEN), Wako-shi, Saitama 351-0198, Japan; and Department of Plant Science, University of Tasmania, G.P.O. Box 252-55, Hobart, Tasmania 7001, Australia (J.L.W., J.B.R.)

Gibberellin (GA) 20-oxidase (GA 20-ox) and GA 3beta -hydroxylase (GA 3beta -hy) are enzymes that catalyze the late steps in the formation of active GAs, and are potential control points in the regulation of GA biosynthesis by light. We have investigated the photoregulation of the GA 20-ox and GA 3beta -hy transcript levels in pea (Pisum sativum L.). The GA 20-ox transcript level was higher in light-grown seedlings than in etiolated seedlings, whereas GA 3beta -hy mRNA accumulation was higher in etiolated seedlings. However, transfer of etiolated seedlings to light led to a 5-fold increase in the expression of both transcripts 4 h after transfer. GA 20-ox mRNA accumulation is regulated by both phytochromes A and B. Transfer to light also resulted in a 6-fold decrease in GA1 levels within 2 h. These results suggest that the light-induced drop in GA1 level is not achieved through regulation of GA 20-ox and GA 3beta -hy mRNA accumulation. The application of exogenous GA1 to apical buds of etiolated seedlings prior to light treatments inhibited the light-induced accumulation of both GA 20-ox and GA 3beta -hy mRNA, suggesting that negative feedback regulation is an important mechanism in the regulation of GA 20-ox and GA 3beta -hy mRNA accumulation during de-etiolation of pea seedlings.

1 This work was supported by the Frontier Research Program (RIKEN).

2 Present address: Department of Molecular Genetics, Cambridge Laboratory, John Innes Centre, Colney Lane, Norwich NR4 7UJ, UK.

* Corresponding author; e-mail aitali{at}bbsrc.ac.uk; fax 44-1603-505725.

© 1999 American Society of Plant Physiologists


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